z*******6
发帖数: 679 | 1 为什么每次发帖都没人理。。。文风不合？？？。。。 |
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L**********O
发帖数: 1761 | 3 what do you wanna ask about MSC? |
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J******s
发帖数: 7538 | 5 pretty difficult, unless u have quite good mouse model, e.g. KO mice which
have specific phenotype. if ur boss has no publication or promising
preliminary date on this, do not touch on it, do sth easier.
are u choosing Ph.D boss? if yeah, please rethink it |
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m*p
发帖数: 226 | 6 Fibroblast in which organ or tissue? EMT and MET can happen in a lot of
tissues or organs. |
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C**S
发帖数: 522 | 7 他这个模型发没发过文章呢？没有的话你去了是不是就捡个第一作者了？如果发了，是
多大的文章，大文章的话，可能可以很容易就引申出几篇一般的文章。 |
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L**********O
发帖数: 1761 | 8 MSC is not a good stem cell... |
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z*******6
发帖数: 679 | 9 没有太大的paper，就是十几分的。。。感觉是模型刚刚建起来，他就独立出来自己搞
了。。。 |
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z*******6
发帖数: 679 | 10 我也就是担心这个。。。
不过不是说已经应用于临床了吗？ |
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w***m
发帖数: 10498 | 11 Speaking mesenchymal stem cell only, this is a very interesting area. I
remember Bob Weinberg (whitehead) had a nice review or research article
published in Nature few years ago that has something to do with Bone marrow
derived MSCs.
Talking about your career, it's totally different issue. If you want to
build your career on something big, you should pick something hard to start.
However, sounds to me that you haven't done to much reading yet. If you
want to build your career in a good way, you ... 阅读全帖 |
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z*******6
发帖数: 679 | 12 嗯，感谢你中肯的意见，我对hard的东西并不惧怕，怕的就是最后什么都捞不到，那就
挺郁闷的，而且是个新PI，connection之类的肯定没有我rotate的另外一个lab好，现
在就在这两个中间犹豫，那个lab，即使时间长一点，6-7年，可是老板算是个牛，而且
基本怎么能保证一篇blood一篇jbc的水平；而这个新lab，基本没有任何保障了。。。
嗯，是啊，确实没有读什么paper，也是刚进这个lab rotation了一周，觉得各方面都
很喜欢，各种感觉都很好。。。有点想定下来了。。。所以关心大家对这个领域的看法
，因为我毕竟是个菜鸟，就算读上几篇paper，也跟大家比差很远，心里也是很玄乎的
。。。所以了。。。
marrow
start. |
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m***c
发帖数: 177 | 13 En, you are fairly honest person! A very traditional dilemma, big lab and
small lab versus good ideas and average ideas. Ok, let me ask you another
question, where does the new guy come from, I mean, from a very well known
lab and was very well trained or just from an average environment? I am not
saying there is no genius from average environment, but chance will be lower
. |
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z*******6
发帖数: 679 | 14 给你站内信了，麻烦check一下。。。
and
another
known
not
lower |
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z*******6
发帖数: 679 | 15 给你站内信了，麻烦check一下。。。
EMT |
|
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z*******6
发帖数: 679 | 17 我考。。。太犀利了。。。
直接把我爆了。。。
就这个，其他的貌似都是这个基础上的。。。
既然直接人肉到了，我也不介意，大家觉得有的做吗？ |
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c****l
发帖数: 1086 | 18 不好意思，没有暴露你的意思，也没有人肉的兴趣，
我只是本身对PDGFRA的功能，以及MSC 二者都感兴趣，这个paper刚刚出来的时候很是
兴奋了一阵，我们主要是对其在脑子里的功能感兴趣，PDGFRA在脑子里比较特异的在
oligodendrocytes progenitor cells 里表达， 在glioblastoma里有amplification，
所以我比较关注。
Philippe Soriano很牛的。有前途！ |
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z*******6
发帖数: 679 | 19 为什么每次发帖都没人理。。。文风不合？？？。。。 |
|
|
L**********O
发帖数: 1761 | 21 what do you wanna ask about MSC? |
|
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m*p
发帖数: 226 | 23 Fibroblast in which organ or tissue? EMT and MET can happen in a lot of
tissues or organs. |
|
C**S
发帖数: 522 | 24 他这个模型发没发过文章呢？没有的话你去了是不是就捡个第一作者了？如果发了，是
多大的文章，大文章的话，可能可以很容易就引申出几篇一般的文章。 |
|
L**********O
发帖数: 1761 | 25 MSC is not a good stem cell... |
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z*******6
发帖数: 679 | 26 没有太大的paper，就是十几分的。。。感觉是模型刚刚建起来，他就独立出来自己搞
了。。。 |
|
z*******6
发帖数: 679 | 27 我也就是担心这个。。。
不过不是说已经应用于临床了吗？ |
|
w***m
发帖数: 10498 | 28 Speaking mesenchymal stem cell only, this is a very interesting area. I
remember Bob Weinberg (whitehead) had a nice review or research article
published in Nature few years ago that has something to do with Bone marrow
derived MSCs.
Talking about your career, it's totally different issue. If you want to
build your career on something big, you should pick something hard to start.
However, sounds to me that you haven't done to much reading yet. If you
want to build your career in a good way, you ... 阅读全帖 |
|
z*******6
发帖数: 679 | 29 嗯，感谢你中肯的意见，我对hard的东西并不惧怕，怕的就是最后什么都捞不到，那就
挺郁闷的，而且是个新PI，connection之类的肯定没有我rotate的另外一个lab好，现
在就在这两个中间犹豫，那个lab，即使时间长一点，6-7年，可是老板算是个牛，而且
基本怎么能保证一篇blood一篇jbc的水平；而这个新lab，基本没有任何保障了。。。
嗯，是啊，确实没有读什么paper，也是刚进这个lab rotation了一周，觉得各方面都
很喜欢，各种感觉都很好。。。有点想定下来了。。。所以关心大家对这个领域的看法
，因为我毕竟是个菜鸟，就算读上几篇paper，也跟大家比差很远，心里也是很玄乎的
。。。所以了。。。
marrow
start. |
|
m***c
发帖数: 177 | 30 En, you are fairly honest person! A very traditional dilemma, big lab and
small lab versus good ideas and average ideas. Ok, let me ask you another
question, where does the new guy come from, I mean, from a very well known
lab and was very well trained or just from an average environment? I am not
saying there is no genius from average environment, but chance will be lower
. |
|
z*******6
发帖数: 679 | 31 给你站内信了，麻烦check一下。。。
EMT |
|
|
z*******6
发帖数: 679 | 33 我考。。。太犀利了。。。
直接把我爆了。。。
就这个，其他的貌似都是这个基础上的。。。
既然直接人肉到了，我也不介意，大家觉得有的做吗？ |
|
c****l
发帖数: 1086 | 34 不好意思，没有暴露你的意思，也没有人肉的兴趣，
我只是本身对PDGFRA的功能，以及MSC 二者都感兴趣，这个paper刚刚出来的时候很是
兴奋了一阵，我们主要是对其在脑子里的功能感兴趣，PDGFRA在脑子里比较特异的在
oligodendrocytes progenitor cells 里表达， 在glioblastoma里有amplification，
所以我比较关注。
Philippe Soriano很牛的。有前途！ |
|
D***y
发帖数: 693 | 35 你这个olson很牛的，他phd 也是从大实验室出来的，有篇cell. |
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m*p
发帖数: 226 | 36 I watched one of the senior author's interviews, and she means all the cells
were removed.我认为是那个记者写错
了.
Actually, heart and vessels have abundant of ECM, and I think the "heart
frame" or the "scaffold" is the ECM.
Some people say about 75% of the cells in the heart are fibroblasts,
although their volume contributes to only
15% of the heart, and the fibroblasts secret lots of ECM, and if fibrosis
happens, there will be even more ECM.
That might explain why all cells were removed, but there is a h... 阅读全帖 |
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G*******s
发帖数: 92 | 37 Expert Opin Investig Drugs. 2011 May;20(5):657-67. Epub 2011 Mar 24.
Lysophosphatidic acid-1-receptor targeting agents for fibrosis.
Rancoule C, Pradère JP, Gonzalez J, Klein J, Valet P, Bascands JL,
Schanstra JP, Saulnier-Blache JS.
email: N***********[email protected]
Thanks! |
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n******u
发帖数: 418 | 38
in
从我目前看的paper来说，EMT包括developmental EMT,fibrosis and wound EMT和
cancer
EMT，所以发育阶段的那个例子也是一种EMT，只不过它和cancer EMT不同的是，这个是
tightly
regulated的
至于in vivo model我现在也看得一头雾水。。。感觉只要是epithilial cell line都
可以拿来
做model。。。 |
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a********k
发帖数: 2273 | 39 花了一个小时，深深的鄙视一下自己的无聊行径！！
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branches in lamellipodia. Cell. 2008 Sep
5;134(5):828-42. PubMed PMID: 18775315; PubMed Central PMCID: PMC2570342.
Cai L, Makhov AM, Bear JE. F-actin... 阅读全帖 |
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M*****n
发帖数: 16729 | 40 knock-out piggy has been successfully generated.
pig is an ideal model for cystic fibrosis.
模型的优劣 |
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d***y
发帖数: 8107 | 41
这个名词cystic fibrosis对我来说太专业了……………… |
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M*****n
发帖数: 16729 | 42 I forgot the name of PI,
last year I heard the seminar about pig model of cystic fibrosis.
it is either U of Iowa or U of indiana. |
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s******y
发帖数: 220 | 43 我的实验目的是看小鼠心脏是否有heart failure相关的remodeling。
查paper一般看到大家就做H&E staining以及 masson trichrome staining看是否有
fibrosis。 是不是通常做这两个就够了？
还有就是准备sample的时候，需要多少tissue呢？ 比如一颗心的话是不是切下一个横
截面的tissue就足够了。 如果打算节约点的话，半颗心够不？
谢谢了。 |
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w******y
发帖数: 2504 | 44 看心衰的话，这些指标是不行的吧。这些指标只能说明HYPERTROPHY的吧？一般来说，
就是切个2毫米厚的横切面来看心肌细胞横切面大小和FIBROSIS。
要看心衰的话，估计还得作ECHO才行。不晓得还有什么蛋白或是其他的MARKER没有。 |
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n****n
发帖数: 295 | 45 直接联系她吧。她自己写的自我介绍如下：
I am a Ph. D. graduated from Ocean University of China, a highly reputable
university in China. As a Ph. D. trained in microbiology, biochemistry,
molecular biology field, my research focused on investigating one kind of
marine bacterium, Pseudomonas fluorescens, including the screening and
identification of bacterial strains, fermentation, purification and
application of enzyme, recombinant enzyme expression and study of the
enzymatic degradation of bacterial alginate obtai... 阅读全帖 |
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w******y
发帖数: 2504 | 46 Could anyone do me a favor sending a PDF copy of the following paper to my
email p***********[email protected]?
J Hypertens. 2006 Aug;24(8):1663-70.
Inhibition of mTOR reduces chronic pressure-overload cardiac hypertrophy and
fibrosis.
Gao XM, Wong G, Wang B, Kiriazis H, Moore XL, Su YD, Dart A, Du XJ.
Thanks so much. |
|
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n***3
发帖数: 663 | 48 Nat Rev Gastroenterol Hepatol. 2014 Feb;11(2):74. doi: 10.1038/nrgastro.2013
.244. Epub 2013 Dec 10.
Liver: Hepatic stellate cells hold the key to liver fibrosis.
Ray K.
Please send the paper to e*********[email protected]
Thanks! |
|
y****i
发帖数: 455 | 49 Nat Rev Nephrol. 2014 Feb 18. doi: 10.1038/nrneph.2014.21. [Epub ahead of
print]
Genetics: Epigenetic reversal attenuates renal fibrosis.
Holmes D.
PMID: 24535424 [PubMed - as supplied by publisher]
email: y****[email protected] |
|
n**********u
发帖数: 77 | 50 Siderophore synthesis by mucoid Pseudomonas aeruginosa strains isolated from
cystic fibrosis patients
Barbara Haas, Eileen Murphy, Domenic Castignetti
Canadian Journal of Microbiology, 1991, 37(8): 654-657, 10.1139/m91-111 |
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