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全部话题 - 话题: epitype
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J**n
发帖数: 1224
1
来自主题: Biology版 - Re: 有关免疫组化的问题
That's not surprising at all. I will be very surprised if it was the
other way around.
Monoclonal antibody is against one specific epitype of antigen. If
it works, it works. If not (for example, under immunohistochemistry
conditions, methanol, paraglutahyde...), it won't. Polyclonal antibody
is against a great variety epitype of the antigens. Some clones of
antibody might only work for western, the others might work for immunohist,
some might work for flow, eta. Very few monoclonal antibodies ca
n******7
发帖数: 12463
2
对这个领域刚接触,近未来最主流技术的应该是bisulfite sequencing吧
我感觉这个就是bisulfite treatment + NGS
其可能的问题应该都跟bisulfite treatment有关,比如转化不完全之类
还有什么需要注意的吗?
另外,其他一些技术值得关注吗?比如基于mass spectrometry的 (SEQUENOM
EpiTYPER)?
谢谢
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