y******8
发帖数: 1764 | 1 how about Protein G B1 domain and binding motif on Fc |
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y******8
发帖数: 1764 | 2 Don't know the size limits. If the fusion domain can go above 25kd, then GST
is a very good choice. |
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y******8
发帖数: 1764 | 3 The protein G B1 domain is about 8kd, and would be hard to synthesize.
Pratically, this is the only reason you need to ask for the material and
permission to start. Otherwise, you could just do it and wait for response.
Usually, people don't pay attention to nonprofit stuff. |
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s******y
发帖数: 28562 | 4 小声地再问一句：
没有人知道么？
我其实要找的就是两个有比较高结合能力的蛋白domain，最好是长度都
不超过50个氨基酸的，而且序列已知的，而且是heterodimer.
which |
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i******k
发帖数: 4625 | 5 Try membrane lipid blot first (companies like Echelon provides different
Membrane Lipid Array and Strips)
then make liposomes to confirm the binding ability
meanwhile, this domain is likely to have some similarity in sequence to some
known lipid binding domains. |
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K**4
发帖数: 1015 | 6 很难说，大多数的protein都是multi－domain的
而这个问题对于不同物种可能也答案不同
那些进化位置很早的物种，可能蛋白组成上会简单些
所以总的来说，估计有70－80％是multi－domain的吧
如果基于计算的结果，基于pfam中的数据肯定是underestimate的 |
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p***g
发帖数: 66 | 7 我从文献中得到了一些疾病相关的SNP(single point mutations, for example,
rs85462)和对应Gene. 我想看看这些SNP都是什么类型: frameshift, missense,
nonsense etc. 该查什么database, db.snp?
然后我想作图看看mutations 分布在哪些protein domain (象附件图). 需要用什么
databases (protein domain mapping) 和什么软件. 请各位多多指点, 万分感激!
A little more information: I need to check several hundreds of SNPs for tens
of genes. Just visualize their distribution and effect on protein function/
structure. That’s why I need some software/web applications to do it. |
|
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f*******a
发帖数: 80 | 9 Hi Folks, Does someone know a book on optimization or estimation over
complex domain? The books I have in hand carry out the analysis in the real
domain only. However, the problem I am working on uses phasor/cplx nmber. I
am from time to time confused on the use of transpose and conjugate
transpose.
Thank you. |
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a*i
发帖数: 1652 | 10 what is the relationship between domain size vs molecular weight in block
copolymers? Does the domain size change much with proportional increase of
molecular weight? Thanks. |
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m****6
发帖数: 79 | 11 在data science这一块这个domain knowledge只是指software engeering相关的呢？还
是说我有其它领域的知识，比如heathcare，或者说marketing，也可以说有了domain
knowledge？
兄弟我身在苦逼的专业，正考虑转行的可能性。 |
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w*******y
发帖数: 60932 | 12 GoDaddy.com offers a new 1-year domain name registration for 99 cents via
coupon code "FALL99" . An 18-cent ICANN fee applies. This offer isn't
limited to .com domains; .org, .net, .biz, and others are also eligible.
Link:
http://www.godaddy.com
|
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w*******y
发帖数: 60932 | 13 GoDaddy.com, the world's largest domain name registrar, is offering Buy.com
customers an exclusive promotion
Buy a domain name for only $1.00 by clicking on the link or entering promo
code GOBBLE in your cart.
Click Here
*See landing page for details.
|
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w*******y
发帖数: 60932 | 14 GoDaddy.com is offering $1.49 domain names with the coupon code NEWYEAR11
Domains included are: COM, NET, ORG, BIZ, US, MOBI, CA, CO.UK, IN
|
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w*******y
发帖数: 60932 | 15 GoDaddy 30% off .COM domains, including renewals. Expires Jan 16th.
(Also took 30% off of my .INFO renewal as well)
Use code gdz1112z
Just used it on six 1-year domain renewals.
$0.18 ICANN fee applies.
|
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w*******y
发帖数: 60932 | 16 Due to the controversy brewing from Godaddy's CEO, Bob Parson's, shooting an
elephant, Namecheap.com is offering $4.99 domain transfers (limit 10 per
user)
quote
Show your protest by saying BYEBYEGD again and transfer your domains to
Namecheap for $4.99 for the next 24 hours through 11:59pm EST on 3/31/11 (
limit 10 per user). |
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w*******y
发帖数: 60932 | 17 Link:
http://www.facebook.com/NameCheap
"Like" Namecheap on Facebook, and if we meet the threshold by
July 08, 2011, this offer will become available:
$1.99 .com/.net/.org registration
Regular Price: $10.16
Valid for first year registration, 1 domain per account
Each domain name comes with free WhoisGuard Privacy Protection ($2.88 value)
and free email forwarding.
4,665 likes at the time of this posting so let's make it happen!
|
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w*******y
发帖数: 60932 | 18 Link:
http://www.namecheap.com/promos/2011/black-friday-cyber-monday-
Namecheap.com will be releasing crazy Black Friday and Cyber Monday deals.
These deals will be released on November 25th and 28th, 2011 only at set
intervals to give everyone a chance to participate. Stay tuned by watching
this page and celebrate the start of the holiday season with massive savings
$0.99 DOMAIN TRANSFER
VALID FOR .COM/ .NET/ .ORG and .BIZ TRANSFERS
Max. 5 domains per customer at this price for the whole promot... 阅读全帖 |
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w*******y
发帖数: 60932 | 19 Just for anyone interested, IMeanWebHosting's "NoSopa" coupon code still
seems to be working, even after SOPA is squashed and domains went up
everywhere. Go to Link:
https://www.imeanwebhosting.com/secure/billing/cart.php
, add the basic plan to the cart on a yearly anual basis, choose your
domain, checkmark whois ID protection if you want it (godaddy charges $6.99
for that alone), and then enter "NoSopa" at checkout.
|
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w*******y
发帖数: 60932 | 20 Starting at 12:00am EST, March 6th Namecheap will be offering $1.99 COM/NET/
ORG domain registration/transfer. Duration of the promotion is 24 hours.
Limit 1 per account. Multiple accounts not allowed.
Check for additional details on their Facebook page Link:
https://www.facebook.com/NameCheap
.
quote
Hey everyone - as the Current Deal page states, this deal will NOT
be active before March 6th. Until then, you will NOT be able to order the
domain at $1.99.... 阅读全帖 |
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G*******s
发帖数: 10605 | 21 【 以下文字转载自 SmartShopper 俱乐部 】
发信人: GoRockets (火箭加油), 信区: SmartShopper
标 题: $1.99 GoDaddy .com new or transfer domain
发信站: BBS 未名空间站 (Wed Nov 13 12:57:20 2013, 美东)
GoDaddy is offering $1.99 price for the first year of one new or transfer .
COM per customer. Your discount will be applied in your shopping cart. Plus
ICANN fee of $0.18 per domain name per year.
http://userquote.com/forum/index.php?topic=2111.msg2465#msg2465 |
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f*******g
发帖数: 1290 | 22 eminent domain早就是settled law了，政府需要拆，老百姓只能获得补偿，没人敢做
钉子户，钉子户在美国是死路一条，抓你没商量，路过的连个侧目的都不会有。 |
|
m*****5
发帖数: 23482 | 23
private sector的 eminent domain 比较难通过，但通过以后就是玩真的了
当年纽约建boa大楼的时候就有这么一个故事 |
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k*******p
发帖数: 8821 | 24 【 以下文字转载自 Soccer 讨论区 】
发信人: zlight (Eren Derdiyok), 信区: Soccer
标 题: 想给我的足球公司注册domain ，有什么比较好的deal?
发信站: BBS 未名空间站 (Wed Apr 12 22:58:13 2017, 美东)
哪里有比较便宜的web hosting + one page web space?
谢谢大家
名字一开始想是沪鲁足业，所有人说听起来像洗脚店 |
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g******t
发帖数: 11249 | 25 programming属于skills，更新很快，尤其是互联网行业
专业知识属于domain knowledge，是百多年的积累，需要长时间的训练
看耗子老师，8年耗出来一个arizona的光学博士
就算家里蹲了好些年，还是有大公司正职要
互联网就很难了
特别这年头身份又不好办 |
|
发帖数: 1 | 26 说得跟你有domain knowledge似的 |
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z****u
发帖数: 255 | 27 政府工程的eminent domain和私人的差别大着呢。不能比。 |
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w*s
发帖数: 7227 | 28 刚从出租房回来，明天还要修车，一个人带孩子，周末还要带父母出去转转，上班的东
西2周没进展了压力很大。。。
你们把domain name, linux web host搞好，我装discuz。 |
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w*s
发帖数: 7227 | 29 我老说的是你们去订个linux web host company, 比如1and1, godaddy,
（查下discuz的安装要求）
再买个domain name |
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h****a
发帖数: 883 | 30 想做个网站，哪个domain好用呢？最好是不被国内屏蔽的。谢谢 |
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s****y
发帖数: 18 | 31 tech implementation 说不出个所以然
不过user experience 感觉没什么区别，不过可能独立domain的话 以后方便将服务分
在不同的server上。 |
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t******g
发帖数: 1667 | 32 比如了解业务流程、系统架构、协议规范等等，这些东西本来跟CS关系不大或者没啥关
系的，但是又必须的了解。发现我现在大部分时间在这些domain knowledge上面，是不
是不利于将来职业发展？ |
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y********0
发帖数: 371 | 33 小弟在国内做过几年CIM（computer integrated manufacturing),有几年相关经验。现
在找工作求教，到底是去做java developer(在学校两年学了很多java）还是继续做我
有点domain knowledge的老本行？
欢迎多多指教，随便说点啥也受用啊！ |
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S*******u
发帖数: 727 | 34 我想注册一个DOMAIN。我在1&1看到时现在最优惠的 1个月0.99，这样一年就时11.88。
但是1年后就得每月7.99. 你们有无经验的？1年后是不是可以取消？ |
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v*****u
发帖数: 1796 | 35 domain一般按年购买，你的价格看起来像是hosting的价格。 |
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u***n
发帖数: 21026 | 36 .com域名一直是10几刀一年，找大注册商就好了，续费也这个价，你不给钱domain就收
回了，没人给你寄账单，索南就是索南，一点屁事咋呼的要死，我每年给自己域名续费
，10几刀算个毛啊，一年少去一次buffet就有了 |
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S*******u
发帖数: 727 | 37 我上面得LINK上都给出了1%1那家。
是按年算得，但是看到后面也写了12月后，是xx/MONTH 得，这样一年就快100了。
我就怕人家到时AUTO RENEW了，毕竟卡号给人家了。
我以前SUBSCRIBE过2个东西RENEW时都是不通知我，说是AUTO RENEW，而且都是高价
RENEW得。
所以小心点是必要的。
反正我现在注册了一个12月免费得DOMAIN，先用着。 |
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u***n
发帖数: 21026 | 38 都告诉你去Godaddy，namecheap了，你怎么还那么一根经的要用1and1
1and1也是大户，promo的时候1刀注册com，然后快到11个月转走
你要一根经我也没有办法，1and1绝对不会乱收费，也不会按月收domain fee
你有偏执症，赶紧去看心里医生，这个不是100块钱的问题，弄不好上万都治不好。 |
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z**********i
发帖数: 12276 | 39 5年前,因为便宜,申请了一个,结果8月份被自动CHARGE,那个信用卡没有关掉,但一直没
有用.要求REFUND,很痛快,结果发现只给了部分REFUND,因为几块钱,还要付LATE
PAYMENT.
如果有不用的SMALL BUSSINESS DOMAIN,要小心,或提前关掉.否则可能引起不必要的麻
烦.
个人教训,供借鉴. |
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l***s
发帖数: 259 | 40 thanks, do i need to register domain elsewhere or slicehost does both |
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y*****u
发帖数: 159 | 41 Probably best to register a different domain name. |
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b******y
发帖数: 9224 | 42 people only remember .com domain names. So, better to find a name with .com
extension. |
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b******y
发帖数: 9224 | 43 As some of you probably know, I am doing a website that is a question and answer site much like baidu zhidao. Currently my website is using my first name as part of the website. I did it because I couldn't find a good name otherwise.
But, I want to register a new name for my website, ideally the following:
1) It is by Chinese pinyin, because I will target the site for Chinese people
2) It is short, better just two words or three words.
However, any domain name I can think about have been registe |
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p**p
发帖数: 10318 | 44 people
IF chinese means those in mainland, yes, pinyin matters alot. if for
overseas, not necessary.
use I or E, the two letters are hot, like Iphone, Ipod, Ehomnoey, Ebay,
IanswerU.com, UreplyMe.com, IaskU.com, something like that,
Yeah, that happens, do not have expetation on finding an avaiable ideal
domain, That is not gonna happen. You have to compromize, Like add E or I,
or My, Or Your,
might still be available?
see above, that is my personal experience. |
|
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g********g
发帖数: 2172 | 46 心痛不已啊. 单个字的Domain. 具体不好意思说. 不过只好努力做了. 和和. |
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J***e
发帖数: 142 | 47 我们也花了6500美元买了个domain name. 而且都为此高兴. |
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g********g
发帖数: 2172 | 48 我不太同意这, 你花很大力气把网站弄好了, 流量弄上去了, 品牌建立了, 换个DOMAIN
很多事情一切从头来. 另外如果酷讯一开始没出名时买kuxun.com可能只要2000刀, 等
他出名了再买20,000刀也拿不下来. |
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L*1
发帖数: 11537 | 49 $7-10/year, domain only |
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b**j
发帖数: 20742 | 50 android好像太长了，可能很多人不会拼。不过我找了半天带gphone的domain,
都给人注了 |
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