w********y
发帖数: 40 | 1 If I want to extract cytoplasma membrane associated protein, which detergent
is better for that purpose?
Thanks! |
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j*****a
发帖数: 658 | 2 Don't quite understand your question.... You want to detect the
phosphorylation of a protein, why not use western? Even if you do ELISA to
see p-protein, no need to sonicate....RIPA is strong enough to get all your
cytosolic and nuclear protein out. Sonication mostly is used to break down
chromatin, DNA or chop down plasma membrane to release cytoplasma membrane
protein....If I am wrong, please someone corrects me.. So I think in your
case, you probably don't need sonication at all.
Also, freez... 阅读全帖 |
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f**g
发帖数: 28 | 3 Hi all. Anyone know how to localize a protein by its sequence? such Nuclear
, cytoplasma, mitochodrion or nuclear membrane sites? Thanks |
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m*****r
发帖数: 1164 | 6 想用western blot做某蛋白膜在cytoplasma和plasma membrane的分布，请问大家推荐
用什么kit或者buffer。谢谢。 |
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x********e
发帖数: 35261 | 7 精子肯定不可能，缺少cytoplasma和线粒体DNA. 卵子可以parthenogenesis. 正常情况
下胚胎无法存活。但是可以用这种方式诱导胚胎干细胞。
不过话说回来，为什么高等动物发展出parental imprinting这个机制？ |
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d********y
发帖数: 176 | 8 i don't think so... and please define what you mean by distribution.
spatial distribution within the membrane? or distribution on the membrane vs
in the cytoplasma? or distribution of transmembrane/peripheral portion?
man... please be specific |
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