n***w
发帖数: 2405 | 1 No. You misunderstood the paper. ATP is used to activate the adenosine
receptor.
The largest calcium store inside the cell is endoplasmic reticulum, which
can release a large amount of calcium upon IP3-R is activated.
Mitochondria is critical in maintaining calcium homeostasis and will take up
calcium if there is calcium overload.
You can transfect your MEFs with cytoplasmic aequorin (you can search on
this to get a better idea), which is widely used as a intracellular calcium
indicator.
You can... 阅读全帖 |
|
s********n
发帖数: 342 | 2 最近在赶一个实验，做了CD4在脑部切片的IS，
信号很好，只是pattern很奇怪，大多数人show的staining是环状的，cytoplasm里表达
罗。
可是我的结果总是整个细胞都有信号，我用了两个不同公司的抗体，包括DAKO，用
confocal时
pinhole也设得很低，应该比核小多了。希望板上高手达人指教。谢谢。
另外，如果做cytokine和receptor的costaining，是不是假设所有cytokine都会bind到
receptor上呀？那这样的结果就是cytokine的staining包括了所有receptor的staining
呀。
恳请指教。 |
|
E**********1
发帖数: 73 | 3
I've checked that paper. Based on my understanding, the paper said ATP
stimulation can temporarily inhibit respiration and reduce mitochondrial
membrane potential, so inhibit mitochondrial Ca2+ uptake. After temporary
inhibition of Ca2+ uptake, ATP is consumed and mitochondrial respiration
resumed and cause mitochondrial Ca2+ boost. And the boost after ATP
stimulation can reflect the ability of cellular respiration.
But this still doesn't solve my problem, which needs to increase cytoplasmic
Ca... 阅读全帖 |
|
Z******t
发帖数: 206 | 4 想分离 nuclear / cytoplasmic 蛋白,没做过 fractionation,
谁能推荐一个效果好的 fractionation 试剂盒啊? |
|
Z******t
发帖数: 206 | 5 请问有什么好的protocol 或者试剂盒吗?
我新手,做fractionation 发现 cytoplasmic fraction 里面有 histone.
这种情况怎么避免阿? |
|
c**y
发帖数: 127 | 6 请教各位大虾，
根据大家经验，觉得哪家公司的
Nucleo-cytoplasmic fractionation kit分离后的fraction比较纯，contamination比
较少？
多谢！ |
|
f**d
发帖数: 768 | 7 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
|
f**********e
发帖数: 6 | 8 hi
Here is my cv, it would be appreciated if I can have the chance to review
the article.
Thanks
Hao
Harvard Medical School
Curriculum Vitae
Name: Hao Wang
Office Address: Department of Cancer Immunology and AIDS
Dana-Farber Cancer Institute
Harvard University
3 Blackfan Circle, CLSB 1010
Boston, MA 02215
Home Address: 103 Gordon street,
Boston, MA 02120
Work Phone: (617) 602-6786
W... 阅读全帖 |
|
l**********1
发帖数: 5204 | 9 Firstly stable isotope 15N label of that protein then inert cell,
after 72 hrs
add synthetic macromolecules to mimic the crowded cytoplasm
next extract nuclei protein
the residue is cytoplasm protein
next do mass spectrometry individually
References:
1,
//www.isotope.com/uploads/file/prot_brochure_lysineupdatelores.pdf
2,
PLoS One. 4:e7560. |(2009)
Isolation of cell nuclei using inert macromolecules to mimic the crowded
cytoplasm.
http://www.ncbi.nlm.nih.gov/pubmed/19851505 |
|
G****e
发帖数: 11198 | 10 【 以下文字转载自 Biology 讨论区 】
发信人: Evangeline4 (Evangeline4), 信区: Biology
标 题: 两篇一模一样的文章(2012, 2015)
发信站: BBS 未名空间站 (Tue Oct 6 13:38:32 2015, 美东)
The microRNA-1246 promotes metastasis in non-small cell lung cancer by
targeting cytoplasmic polyadenylation element-binding protein 4
(Weihua Huang, Huifen Li, and Rongcheng Luo, 2015, Diagnostic Pathology
Journal)
MicroRNA-550a acts as a pro-metastatic gene and directly targets cytoplasmic
polyadenylation element-binding protein 4 in hepatocellular carci... 阅读全帖 |
|
S******i
发帖数: 71 | 11 【 以下文字转载自 Biology 讨论区 】
发信人: Suozucai (Zuzu), 信区: Biology
标 题: 南方医科大学中西医结合医院院长罗荣城教授严重剽窃他人论文，发表到diagnostic pathology.
发信站: BBS 未名空间站 (Wed Oct 7 21:14:58 2015, 美东)
南方医科大学中西医结合医院院长罗荣城教授严重剽窃他人论文，发表到diagnostic
pathology.
见过造假的，没见过这么造假的。。。。。。
论文公司的科学家学习学习吧，一本万利
The microRNA-1246 promotes metastasis in non-small cell lung cancer by
targeting cytoplasmic polyadenylation element-binding protein 4 (Weihua
Huang, Huifen Li, and Rongcheng Luo, 2015, Diagnostic Pathology
Journal)
MicroRNA-550a acts as a pr... 阅读全帖 |
|
H********g
发帖数: 43926 | 12 These natural barriers can be overcome in a laboratory, at least at first.
Such attempts typically result in a hybrid embryo which dies after a few
cell divisions.
For example, the egg's protective covering can be stripped off, allowing the
sperm of the other species access to the egg. Sperm can also be injected
directly into the egg. When this is done, fertilization occurs. The
resulting hybrid embryo is not viable, however. It typically goes through
one cell division, resulting in a two-cell e... 阅读全帖 |
|
S***n
发帖数: 102 | 13 CMS genes were found but not by Rice Breeder Yuan. See
Chase, C.D. (2006). Cytoplasmic male sterility: a window to the world of
plant mitochondrial-nuclear interactions. Trends Genet. 23, 81-90.
Schnable, P.S., and Wise, R.P. (1998). The molecular basis of cytoplasmic
male sterility and fertility restoration. Trends Plant Sci. 3, 175-180. |
|
a***e
发帖数: 1010 | 14 your procedure has some problems. (1) the first NP-40 10 min is too long. (2
) the second 2x wash is not enough to remove residue cytoplasm.
think about this way, if you don't care about recovery efficiency:
for the first step, 0.5% NP40 for only 1 min, transfer supernatant to new
tube. this should be very clean cytoplasm.
for the second step, 0.5% NP40 for 10 min, wash 2x, save pellet this time.
this should be very clean nucleus.
I mean 1 min and 10 min are very extreme examples. |
|
k*******c
发帖数: 142 | 15 thanks for your replying.
we are using bacteria. what I found here:
Green fluorescent protein (GFP) is an ideal cytoplasmic marker because it
fluoresces only when located in the cytoplasm (19, 20). When GFP is targeted
to the periplasm through fusion with a membrane-spanning domain (MSD), it
fails to fold properly and does not fluoresce.
what do you think?
thanks again |
|
c********r
发帖数: 189 | 16 Histone H3 不应该主要在nuclei里吗？为啥我做cell fractionation, 分离nuclear和
cytoplasmic fraction, 每次western blot里cytoplasmic fraction的histone H3 最
多。
cell fractionation的问题 还是抗体的问题，还是本来就是如此？郁闷啊。 |
|
c********r
发帖数: 189 | 17 Histone H3 不应该主要在nuclei里吗？为啥我做cell fractionation, 分离nuclear和
cytoplasmic fraction, 每次western blot里cytoplasmic fraction的histone H3 最
多。
cell fractionation的问题 还是抗体的问题，还是本来就是如此？郁闷啊。 |
|
z*t
发帖数: 863 | 18 Beutler得奖，Medzhitov会不会有意见？
http://www.sciencedirect.com/science/article/pii/S1074761309002
Ruslan Medzhitov
This year marks the 20th anniversary of a publication (Janeway, 1989) that
in many ways revolutionized our understanding of the immune system. As part
of the proceedings of the Cold Spring Harbor Symposium on Immune Recognition
, the paper authored by the late Charles A. Janeway, Jr. was not a standard
peer-reviewed publication. In fact, Charlie used to refer to it as “the
best paper I've ... 阅读全帖 |
|
b******s
发帖数: 1089 | 19 在数据分析中，他们会做一些correlation，据说可以去除移动带来的噪音。
我想知道楼上有人说FCS主要使用平衡态体系，如果这样，应当不适用活体细胞，更使
用溶液的环境。
细胞内有复杂的cytoplasmic streaming，并且有大量的cytoplasmic components导致
的运动延迟。 |
|
b******s
发帖数: 1089 | 20 在数据分析中，他们会做一些correlation，据说可以去除移动带来的噪音。
我想知道楼上有人说FCS主要使用平衡态体系，如果这样，应当不适用活体细胞，更使
用溶液的环境。
细胞内有复杂的cytoplasmic streaming，并且有大量的cytoplasmic components导致
的运动延迟。 |
|
x**2
发帖数: 255 | 21 http://www.madsci.org/posts/archives/2002-04/1018464369.Cb.r.ht
The average distance that each type of molecule travels from
its starting point is proportional to the square root of the time involved:
that is, if it takes a particular molecule 1 second on average to go 1 um,
it will go 2 um in 4 seconds, 10 um in 100 seconds, and so on. Diffusion is
therefore an efficient way for molecules to move limited distances but an
inefficient way for molecules to move long distances.
Experiments performe... 阅读全帖 |
|
k****s
发帖数: 1209 | 22 原文在
http://www.skizit.biz/2013/07/21/ecdysone-insect-hormone-an-ind
Ecdysone Insect Hormone Switches on Disease
21 Jul 2013 | ECDYSONE
MORGELLONS:
INSECT HORMONE ECDYSONE TRIGGERS GENETIC CHANGES
TO BUILD NEW HAIR, SKIN, NAILS and CAUSE DISEASES
SkizitGesture-2013
Morgellons is a military grade entomological terror weapon being used to
torture and kill innocent civilians. The medical community has not been
informed about this disease and its complexity keeps it from being properly
identified, ... 阅读全帖 |
|
h**********r
发帖数: 671 | 23 做微生物的人说说：pyruvate还是会利用pdh产生acetyl-CoA,但是这是在mitochondria
里的，不能直接合成lipid。然后利用部分的TCA cycle转化成citric acid,这个可以进
入cytoplasm.在citric acid lyase作用下合成acetyl-CoA。TCA cycle可以部分的shut
down,甚至可以有reverse,保证大量的碳源进入到lipid的合成。同时有NADPH的产生。
前段时间炒的很火的IDH，其实和这个或许有联系。也许在cytoplasm有pdh bypass产生
acetyl-CoA，不是主要来源。acetyl-CoA产生manly CoA是合成脂肪酸的第一步，由ACC
催化完成。这个受很多调控，例如citric acid的诱导，和磷酸化调控（AMP, AMPK）。
pyruvate mitochondria carrier不是找到了一部分了么（science还是nature背靠背文
章）?好像knockout对lipid没啥太大的影响。 |
|
s******y
发帖数: 28562 | 24 关于常山对疟原虫的疗效，正好今年在 Science Translational Medicine 上面有一篇
文章。
应该指出的是，常山即使在中药里，也是被视为一种虎狼之药的，因为其副作用太大，
属于在迫不得已的情况下才会用的药（疟疾在古代算是迫不得已的病情了）。但是它对
疟原虫的确是有效果的，这个也是不能否认的。葛洪收集的那个方子里，42个方子中有
14个还是13个是用这个常山的，这个可以说明那些方子并不是完全胡说八道的。
http://stm.sciencemag.org/content/7/288/288ra77.abstract?sid=7f
The cytoplasmic prolyl-tRNA synthetase of the malaria parasite is a dual-
stage target of febrifugine and its analogs
Abstract The emergence of drug resistance is a major limitation of current
antimalarials. The discover... 阅读全帖 |
|
E*********4
发帖数: 16 | 25 The microRNA-1246 promotes metastasis in non-small cell lung cancer by
targeting cytoplasmic polyadenylation element-binding protein 4
(Weihua Huang, Huifen Li, and Rongcheng Luo, 2015, Diagnostic Pathology
Journal)
MicroRNA-550a acts as a pro-metastatic gene and directly targets cytoplasmic
polyadenylation element-binding protein 4 in hepatocellular carcinoma
(Qi Tian, Linhui Liang, Jie Ding, Ruopeng Zha, Haibing Shi, Qifeng Wang,
Shenglin Huang,Weijie Guo, Chao Ge,Taoyang Chen, Jinjun Li, and ... 阅读全帖 |
|
E*********4
发帖数: 16 | 26 The microRNA-1246 promotes metastasis in non-small cell lung cancer by
targeting cytoplasmic polyadenylation element-binding protein 4
(Weihua Huang, Huifen Li, and Rongcheng Luo, 2015, Diagnostic Pathology
Journal)
MicroRNA-550a acts as a pro-metastatic gene and directly targets cytoplasmic
polyadenylation element-binding protein 4 in hepatocellular carcinoma
(Qi Tian, Linhui Liang, Jie Ding, Ruopeng Zha, Haibing Shi, Qifeng Wang,
Shenglin Huang,Weijie Guo, Chao Ge,Taoyang Chen, Jinjun Li, and ... 阅读全帖 |
|
S******i
发帖数: 71 | 27 南方医科大学中西医结合医院院长罗荣城教授严重剽窃他人论文，发表到diagnostic
pathology.
见过造假的，没见过这么造假的。。。。。。
论文公司的科学家学习学习吧，一本万利
The microRNA-1246 promotes metastasis in non-small cell lung cancer by
targeting cytoplasmic polyadenylation element-binding protein 4 (Weihua
Huang, Huifen Li, and Rongcheng Luo, 2015, Diagnostic Pathology
Journal)
MicroRNA-550a acts as a pro-metastatic gene and directly targets cytoplasmic
polyadenylation element-binding protein 4 in hepatocellular carcinoma (Qi
Tian, Linhui Liang, Jie Ding, Ruopeng Z... 阅读全帖 |
|
l******n
发帖数: 9344 | 28 我岳母在美国被诊断查出cancer,医院出具了病理报告。她人现在已经在国内，为了节
省一些时间，需要把美国的病理报告提供给中国的医院作为参考，使她能够尽快地得到
治疗。由于涉及很多的专业词汇，我把并病理报告copy出来，希望专业的人士能够给各
翻译。
非常感谢！
Diagnosis:
Right pleura, biopsy:
poorly differentiated carcinoma with neuroendocrine differentiation. See
note.
Note: Possible primary site includes the lung and remotely, the pancreas.
Clinical correlation is recommended. This case was discussed with Dr. XXX on
12/14/11. My associate, Dr. XXX, has reviewed this case and concurs with
the diagnosis of neuroendocrine ... 阅读全帖 |
|
l******n
发帖数: 9344 | 29 【 以下文字转载自 MedicalCareer 讨论区 】
发信人: longtian (有人的地方,就有江湖), 信区: MedicalCareer
标 题: 救命！！！帮忙翻译病理报告
发信站: BBS 未名空间站 (Fri Dec 23 01:49:45 2011, 美东)
我岳母在美国被诊断查出cancer,医院出具了病理报告。她人现在已经在国内，为了节
省一些时间，需要把美国的病理报告提供给中国的医院作为参考，使她能够尽快地得到
治疗。由于涉及很多的专业词汇，我把并病理报告copy出来，希望专业的人士能够给各
翻译。
非常感谢！
Diagnosis:
Right pleura, biopsy:
poorly differentiated carcinoma with neuroendocrine differentiation. See
note.
Note: Possible primary site includes the lung and remotely, the pancreas.
Clinical correlation is recommended. Thi... 阅读全帖 |
|
s********n
发帖数: 26222 | 30 方舟子在此文中说“虫子不吃的， 人能吃吗”是中国人的质疑， 而美国人是不会问这
样的问题的， 这显然是骗人的。见下文和其中引用的文献， 其中不少是美国人的科研
。
"Comments on the human health impact of Bacillus thuringiensis toxin gene
product in genetically modified crops"
EPA Review Docket Number OPP-00678B
Professor Joe Cummins
Professor Emeritus of Genetics
University of Western Ontario
I have studied the Environment Protection Agency (EPA) document "Bt Plant-
Pesticides Biopesticides Registration Action Document B. HUMAN HEALTH
ASSESSMENT" and comment in the following ... 阅读全帖 |
|
G*****n
发帖数: 3863 | 31 “The androgen receptor (AR), also known as NR3C4 (nuclear receptor
subfamily 3, group C, member 4), is a type of nuclear receptor[6] that is
activated by binding either of the androgenic hormones, testosterone, or
dihydrotestosterone [7] in the cytoplasm and then translocating into the
nucleus. The androgen receptor is most closely related to the progesterone
receptor, and progestins in higher dosages can block the androgen receptor.[
8][9]
The main function of the androgen receptor is as a DNA-... 阅读全帖 |
|
发帖数: 1 | 32 影响生育的一项研究
二战过后，日本军阀集团，还有其他一些犯罪集团，一直有系统地用生物手段进行种族
屠杀。因为我不是搞医学和生物研究的，所以我也没有系统地进行这方面的探究。但我
在网上阅览文章时候，发现一种给蚊子绝育的方法，就是注入沃尔巴克氏体，一种细菌
，利用雄雌蚊，感染和不感染蚊子杂交，产生绝育效果。 那么我就想，既然蚊子可以
产生绝育效果。那么对于人类，会不会也有同样效果，特别是这项研究被加州大学洛杉
矶分校所发展，又被日本特别加以利用。1971年加州大学洛杉矶分校的Janice Yen与A.
Ralph Barr发现被沃尔巴克氏体感染的雄性尖音库蚊的精子与未被沃尔巴克氏体感染
的雌性尖音库蚊的卵子结合的受精卵由于细胞质不兼容而死亡，[7][8]科学界所后加强
了对沃尔巴克氏体的研究。日本产业技术综合研究所的深津武马和东京大学的今藤夏子
，在研究农业害虫绿豆象和昆虫寄生细菌沃尔巴克氏体的关系时发现，沃尔巴克氏体的
基因存在于绿豆象的染色体中，而且沃尔巴克氏体的染色体结构没有遭到破坏，也就是
说，它的基因可以通过绿豆象这一更高级的动物遗传下去。
所以我认为这个军事犯罪集团很可能通过农... 阅读全帖 |
|
发帖数: 1 | 33 影响生育的一项研究
二战过后，日本军阀集团，还有其他一些犯罪集团，一直有系统地用生物手段进行种族
屠杀。因为我不是搞医学和生物研究的，所以我也没有系统地进行这方面的探究。但我
在网上阅览文章时候，发现一种给蚊子绝育的方法，就是注入沃尔巴克氏体，一种细菌
，利用雄雌蚊，感染和不感染蚊子杂交，产生绝育效果。 那么我就想，既然蚊子可以
产生绝育效果。那么对于人类，会不会也有同样效果，特别是这项研究被加州大学洛杉
矶分校所发展，又被日本特别加以利用。1971年加州大学洛杉矶分校的Janice Yen与A.
Ralph Barr发现被沃尔巴克氏体感染的雄性尖音库蚊的精子与未被沃尔巴克氏体感染
的雌性尖音库蚊的卵子结合的受精卵由于细胞质不兼容而死亡，[7][8]科学界所后加强
了对沃尔巴克氏体的研究。日本产业技术综合研究所的深津武马和东京大学的今藤夏子
，在研究农业害虫绿豆象和昆虫寄生细菌沃尔巴克氏体的关系时发现，沃尔巴克氏体的
基因存在于绿豆象的染色体中，而且沃尔巴克氏体的染色体结构没有遭到破坏，也就是
说，它的基因可以通过绿豆象这一更高级的动物遗传下去。
所以我认为这个军事犯罪集团很可能通过农... 阅读全帖 |
|
A********e
发帖数: 354 | 34 【 以下文字转载自 Biology 讨论区 】
发信人: albertsmwk (.)(.), 信区: Biology
标 题: 第一批“青年千人计划”生物类@publication列表@
发信站: BBS 未名空间站 (Thu Aug 18 15:04:16 2011, 美东)
花了一个小时，深深的鄙视一下自己的无聊行径！！
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branche... 阅读全帖 |
|
a*******a
发帖数: 4233 | 35 现在中科院cns被拒的文章就拆开扔那了
cr现在主要是靠追热点和控制发文量来拉影响因子，已经不怎么靠review了。李党生抓
热点的眼光很准。
比方说整整一年前的那期
Jun 2014 Volume 24, Issue 6, Page 641-773
review
No receptor stands alone: IgG B-cell receptor intrinsic and extrinsic
mechanisms contribute to antibody memory
4 cites
Research article
6篇文章，总引用58
Generation of neural progenitor cells by chemical cocktails and hypoxia
Peigang,17 引用
Pachytene piRNAs instruct massive mRNA elimination during late
spermiogenesis
Liu mofang, 23 引用
Discovery of biclonal origin and a n... 阅读全帖 |
|
b********s
发帖数: 9 | 36 Postdoctoral and Technician Positions in the Dinshaw J. Patel lab
in the Department of Biology, SUSTC, Shenzhen.
Dinshaw J. Patel is a member of the United States National Academy of
Sciences. The Patel structural biology lab is set to open on March 1, 2016
in the Department of Biology at the South University of Science and
Technology of China (SUSTC) in Shenzhen. SUSTC, located in picturesque and
environmentally healthy Shenzhen, is a new University that aims to become a
world-leader in Science... 阅读全帖 |
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u*********1
发帖数: 2518 | 37 很多ALS病人最开始的征兆也是肌肉萎缩，比如用钥匙开门发现没力气。
然后无法说话等等，最后一般死于呼吸衰竭。
用症状来判断是不准确的。
我不知道临床如何判断，但我知道2006年发现的ALS的新的syndrome marker是
cytoplasmic inclusion of ubiquitinated TDP-43
我不知道现在临床上是不是检测这个作为确诊的依据 。
吸。 |
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l*h
发帖数: 4124 | 38 their 结肠低分化神经内分泌癌 could be correct, but i am not convinced by the
evidence presented. say the two common markers, what percentage is 部分?
what distribution and percentage is called 灶性? without proper description,
you don't even know whether they saw them on tumor cells or some normal
tissue.
in addition, there is almost no histological grading information. there is a
lot more than 低分化 in grading. what size are the cells? what do the
nuclei look like? what do the cytoplasms look like? |
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l**x
发帖数: 110 | 39 下面这个患者希望能得到版上医生专家的指导意见，危险多大，如何治疗，预后如何？
患者44岁，男，6年前因为肝癌做了肝移植 （符合米兰标准），之后无灾无病好几年。
去年2016年初发现轻微贫血，血色素12，因为当时在服小剂量阿司匹林，于是停服，看
看有没好转。托托拉拉到了年底，还是贫血，血色素10.5。医生说做个内镜。于是在胃
内发现一个肿瘤，内镜医生直觉是GIST。随后CT，但CT却没有异常。4月初第二次胃镜
，GIST依然还在，取样活检表明可能肝癌转移或者是非常罕见的HAC。第二次CT 报告大
小46x35mm，说比较第一次32mm变大了。可能第一次看片医生那天喝多了？肿瘤位置在
Fundus，非常接近GOJ。
AFP好久没有查了，因为肝上的情况一直很好，肝移植前AFP 100多，移植后AFP一直小
于2。上一次至少是2，3年前。也是疏忽了。现在AFP 254. 显然胃上这个要么是转移，
要么是HAC。
下周二医生还要安排一次PETCT，周三见外科医生，估计排除转移外就安排切除了。 求
这里的专家教授指点，这个情况如何是好？下面我贴上最近几个报告。
个人感觉肝移植后5，6年转移到胃实在... 阅读全帖 |
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p*******g
发帖数: 2976 | 40 【 以下文字转载自 Biology 讨论区 】
发信人: albertsmwk (.)(.), 信区: Biology
标 题: 第一批“青年千人计划”生物类@publication列表@
发信站: BBS 未名空间站 (Thu Aug 18 15:04:16 2011, 美东)
花了一个小时，深深的鄙视一下自己的无聊行径！！
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branche... 阅读全帖 |
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a*******2
发帖数: 67 | 41 现有一篇IF零点几的open access小杂志审稿一篇。有需要凑数量的同学可与我站内联
系 （请给出姓名，单位，邮箱和研究方向)。摘要附上。
Title: "New strategy for managing damping-off and root rot disease of
Cucumber caused by Rhizoctonia solani by Seed Soaking in Formula of
Antioxidant with Micronutrients"
Abstract:
Cucumber is one of many susceptible crops to damping off and root rot
disease caused by Rhizoctonia solani. Sclerotia of R. solani can be remain
viable in soil for several years as important source of primary infection,
In our laboratory trails, ... 阅读全帖 |
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O***n
发帖数: 13127 | 42 【 以下文字转载自 Biology 讨论区 】
发信人: albertsmwk (.)(.), 信区: Biology
标 题: 第一批“青年千人计划”生物类@publication列表@
发信站: BBS 未名空间站 (Thu Aug 18 15:04:16 2011, 美东)
花了一个小时，深深的鄙视一下自己的无聊行径！！
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branche... 阅读全帖 |
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c**i
发帖数: 6973 | 43 大问题 indeed! Others only see pettiness on your part.
Except "bairi," many miss the point.
(A) I re-read the original posting (by az9876), which does not describe what
kind of eatery the noodle shop is. Perhaps it is like fastfood-like* and a
tip is unnecessary. In any event, demanding a tip is tactless.
* In other words, a customer take the tray from counter after ordering the
food--and empty the empty tray to a trash bin when finished.
(B) However, the fundamental issue is fairness.
(1) Natalie... 阅读全帖 |
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R******d
发帖数: 5739 | 44
http://en.wikipedia.org/wiki/Sodium
quote
Biological role
This section requires expansion. (May 2012)
Main article: Sodium in biology
In humans, sodium is an essential nutrient that regulates blood volume,
blood pressure, osmotic equilibrium and pH; the minimum physiological
requirement for sodium is 500 milligrams per day.[47] Sodium chloride is the
principal source of sodium in the diet, and is used as seasoning and
preservative, such as for pickling and jerky; most of it comes from
proce... 阅读全帖 |
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H********g
发帖数: 43926 | 45 【 以下文字转载自 Biology 讨论区 】
发信人: lyray (lyray), 信区: Biology
标 题: 人类灵魂就隐藏在DNA末端krypton原子
发信站: BBS 未名空间站 (Wed Aug 26 16:32:06 2015, 美东)
We owe the discovery of this mysterious biogenetic process to our scientist
AOYOO 221.
It had been discovered a long time before that in the human brain were
krypton atoms (a rare gas that you know), and this discovery was a
significant, transcendent one. It was quite simply the key to the OEMBUAW,
MAN'S THIRD FACTOR, which you do not know and which acts as a link be... 阅读全帖 |
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p********f
发帖数: 5148 | 46 WBC are slightly larger than RBC and have grainy cytoplasm. Normal urine
should contain <5 WBC/field at 400¥ magnification. Increased WBC (pyuria
) can occur due to inflammation, infection, trauma, or neoplasia.
Catheterization or collection of voided urine may introduce a few WBC from
the urogenital tract. |
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l*****a
发帖数: 38403 | 47 Point out that science is a wonderful thing, but discuss "natural" processes
like the ones called entropy (definition: disorder in a system, which
resolves to equilibrium--like dust settles) and chaos (definition: inherent
unpredictability) are not thought of as building anything:
The second law of thermodynamics states that the entropy (or disorder) of
the entire universe (a system comprising of ALL matter) must increase with
time. If the universe had always existed, then entropy of the entire
... 阅读全帖 |
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c****t
发帖数: 19049 | 48 ScienceDaily (July 9, 2011) — For the past decade, researchers have tried
to reprogram the identity of all kinds of cell types. Heart cells are one of
the most sought-after cells in regenerative medicine because researchers
anticipate that they may help to repair injured hearts by replacing lost
tissue. Now, researchers at the Perelman School of Medicine at the
University of Pennsylvania are the first to demonstrate the direct
conversion of a non-heart cell type into a heart cell by RNA transfer... 阅读全帖 |
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M****m
发帖数: 2142 | 50 难道英文不是这么表示吗
microtubules are in the cytoplasm? or microfilaments? |
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