b*****l
发帖数: 9499 | 1 【 以下文字转载自 SysBio 俱乐部 】
发信人: bigsail (河马·轩辕三光), 信区: SysBio
标 题: 困惑啊：more details or less?
发信站: BBS 未名空间站 (Fri Jun 18 20:37:25 2010, 美东)
这个问题困惑我整一年了，发出来向各位大牛求教。
当然了，套话先说一遍：一个 model 并不是 details 越多或者越少就越好，而是看多
复杂的 model 恰好可以适应这个 model 所 target 的应用。
现在切入正题：
有些组做 model 的趋势是越来越复杂和具体，横向看，越来越多的 components 被
integrate 进来，各 module 之间的 crosstalking 也越做越多；纵向看，各个
module 也越来越向 molecular level 迈进，同时越来越多地把 spatial info/
locations and diffussion/mass transport 模拟进来。
另一些组则是越做越简单，试图从大量纷繁复杂的模型中找出简单的共性来分析，或者
是从特别复杂的 |
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a**o
发帖数: 20 | 2 多谢了啊，能否发到信箱里 x********[email protected]，多谢了啊
1、Integrin-TGF-beta crosstalk in fibrosis, cancer and wound healing.
Margadant C, Sonnenberg A.
EMBO Rep. 2010 Feb;11(2):97-105. Epub 2010 Jan 15. Review.
2、Transforming Growth Factor-{beta}1 Induces Smad3-Dependent {beta}1 Integr
in Gene Expression in Epithelial-to-Mesenchymal Transition during Chronic Tu
bulointerstitial Fibrosis.
Am J Pathol. 2010 Aug 13. [Epub ahead of print] |
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j**********k
发帖数: 296 | 3 相反，是因为太难，所以敢认真去碰的人很少。mechanistic study不是随便什么
人都能做的，既烧钱又周期长。很多时候还没有定论，全是crosstalk，牵一发动
全身，不太容易purturb one specific signaling。stem cell research也是这
样，一篇篇的好paper，说白了大都也是灌灌observations，鲜有碰触mechanisms
的。有人做stem cell differentiation期间的expressionprofiling，已经很算是
想干些mechanisms的事儿了，那又如何？profiling这种工作，其结果好比给你张
“错误概率未知，可能很大”的地图，除了他们自己人，谁敢拿它去指路？扯远了，
总之俺是尽量回避纯粹的mechanistic study，等天才们做出来的时候，俺在旁边鼓
鼓掌就行了。 |
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h*******1
发帖数: 15 | 4 美国西北大学土木与环境工程系Prof. Yun Wang 招收联合培养博士生
Prof. Yun Wang from the Department of Civil and Environmental Engineering at
Northwestern University is seeking for Ph.D. candidates to conduct research
in her lab as exchange students under the joint-training programs sponsored
by China Scholarship Council (CSC). Students with educational background
in Environmental Chemistry, Microbiology, Molecular Biology, or related
majors are encouraged to apply. Successful candidates must have research
experience/i... 阅读全帖 |
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n***w
发帖数: 2405 | 5 Astrocytic-neuronal crosstalk: Implications for neuroprotection from brain
injury.
PMID: 21693140
Please send to n***[email protected]
Thank you! |
|
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a****e
发帖数: 128 | 7 Job Title: Postdoctoral Research Associate at the Renal Division, Department
of Medicine, Washington University in St. Louis
Location: Medical School
Full/Part Time: Full-Time
Regular/Temporary: Regular
Essential Functions:
An opportunity is available immediately for a Postdoctoral Research
Associate position at the Renal Division, Department of Medicine. The main
focus of the ongoing research is to understand the molecular mechanisms of
the glomerular filtration barrier and proteinuria. We a... 阅读全帖 |
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l**********1
发帖数: 5204 | 8 Johns Hopkins too 连tenure都过不了
不是看已有领域的挖掘 还要看新领域的digged out data and results.
please refer:
for example:
Lee Martin, Ph.D.
Professor of Pathology, Neuroscience
//pathology.jhu.edu/department/divisions/neuropathology/faculty-staff.cfm
his group recent paper:
线粒体上的控制 NO 的通道控制着细胞老化和自然死亡的过程
"The mitochondrial permeability transition pore regulates nitric oxide-
mediated apoptosis of neurons induced by target deprivation.” (2011)
J Neurosci. 31: 359-370.
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/2... 阅读全帖 |
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g***s
发帖数: 733 | 9 谢谢，你说不可以是指前面的filter会把后面的信号滤掉？我不用dichroic用bandpass
级联行么？
不过不管什么方法，包括你说得专门仪器，是不是都会有一个荧光光谱overlap的问题
？overlap部分的信号就没办法分开吧，会有crosstalk，我查了一下常用label的光谱
，好像如果同时三个或更多，大多会有很强的重叠，如果滤掉重叠部分，光强就太弱了。 |
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a*q
发帖数: 2109 | 10 405 nm 激发 450 nm 荧光 （例如DAPI）
488 nm 激发 510 nm 荧光 （例如GFP，Alexa 488）
561 nm 激发 570 nm 荧光 （例如mCherry，Alexa 555）
647 nm 激发 670 nm 荧光 （例如Cy5，Alexa 647）
以上为四色同时成像的常见选择，基本没有太多Crosstalk。 |
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n***3
发帖数: 663 | 11 Curr Cancer Drug Targets. 2009 Sep;9(6):748-60.
Crosstalk between epidermal growth factor receptor- and insulin-like growth
factor-1 receptor signaling: implications for cancer therapy.
Please send the paper to e*********[email protected]
Thanks so much! |
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j****1
发帖数: 81 | 12 Thank you very much for the comments. I revised abstract. It's attached
below.
----------------------------------------------------------------------------
----------------------------
Colon cancer is the second highest cause of cancer deaths in the United
States (Markowitz & Bertagnolli, 2009). It is well known that APC (
adenomatous polyposis coli) loss predisposes patients to colon cancer, which
has been primarily attributed to the over activation of Wnt signaling.
Although controversial, can... 阅读全帖 |
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b*****n
发帖数: 1841 | 13 生命科学现在的问题是：冗员太多，添砖加瓦毫无影响力的文章太多。
要扭转这个趋势，生命科学应该主要提倡智力竞争而非工作量竞争。要重点资助能影响
整个生命科学研究的新技术，新方法的发明（比如deep seq，单分子成像），以及能影
响全局的新发现，新观点。
添砖加瓦式的发现，比如多数蛋白的相互作用，pathway间的crosstalk，蛋白结构的解
析，基因组的全敲除，系统的筛选等等，应该列入工程性学科，其发现主要以resource
的方式发表。工程性的研究主要以公司化管理，可以招募很多本科级别的工作人员。如
前所述，能系统研究和解决这些问题的新方法，新技术，新思想应该得到更多资助。
最好的杂志在选择文章时，优先发表新意很高的核心数据，而非高大全的东西。例如两
个蛋白相互作用，只要数据足够可信，不一定非要要求面面俱到，体外，细胞，生物整
体，跟疾病的关系一应俱全。许多文章的核心数据就一个figure，但是由于杂志/
reviewer要求完整story，要求数据完备，许多推论性的数据也要做，导致近年的文章
supplemental data越来越多，工作量越来越大。
正如许多网友说的，多数文... 阅读全帖 |
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l*******r
发帖数: 39279 | 14 完全同意啊
最讨厌crosstalk啥的
resource |
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G********e
发帖数: 528 | 15 说归说，尤其最后一段教育PI说的很对
但是做不到
Science里面80％的politics，只有20％的Science
怎么玩啊
一个东西往左也能走，往右也能走，有些人就能预测哪个地方更有可能是对的。但是做
决定的经常就是politics的问题。Politics是绝对不听Science的。
还有，你这种说法，其实就是废话。就好比国内的学校，开会的时候总是教育学生要有
思考创新。但是创新这种东西并不是教育出来的，而是自己悟出来的。你自己做课题想
到一个东西，也不是别人教你要创新你才去想出来的，是你本来就有那个水平。
生命科学现在的问题是：冗员太多，添砖加瓦毫无影响力的文章太多。
要扭转这个趋势，生命科学应该主要提倡智力竞争而非工作量竞争。要重点资助能影响
整个生命科学研究的新技术，新方法的发明（比如deep seq，单分子成像），以及能影
响全局的新发现，新观点。
添砖加瓦式的发现，比如多数蛋白的相互作用，pathway间的crosstalk，蛋白结构的解
析，基因组的全敲除，系统的筛选等等，应该列入工程性学科，其发现主要以resource
的方式发表。工程性的研究主要以公司化管理，... 阅读全帖 |
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l**********1
发帖数: 5204 | 16 非植物 或哺乳的数据库区别也 植物的也 一样在完善
关键是楼主有无NGS and HMM （Hidden Markov Models 的背景或能找到那种背景的
合作人
pls refer
PMID 23435661
by Van der Does D et al., (2013).
Salicylic acid suppresses jasmonic acid signaling downstream of SCFCOI1-JAZ
by targeting GCC promoter motifs via transcription factor ORA59.
Plant Cell. 25: 744-61.
Abstract:
ignored
In silico promoter analysis of the SA/JA crosstalk transcriptome revealed
that the 1-kb promoter regions of JA-responsive genes that are suppressed by
SA are significantl... 阅读全帖 |
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l******u
发帖数: 936 | 17 It is totally different things. The future direction could be systematically
combining CRISPR/Cas, Cre-lox, and tet-on/off technique to temporally and
spatially switch on and off different genes in specific pathway or study the
crosstalk between pathways using the methods, the candidate genes/pathways
should be from the data mining using the public human omics data base, for
example TCGA, ICGC ...... |
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b******j
发帖数: 361 | 18 FGF, TGFβ and Wnt crosstalk: embryonic to in vitro cartilage development
from mesenchymal stem cells
DOI: 10.1002/term.1744
谢谢 |
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b******j
发帖数: 361 | 19 【 以下文字转载自 Immigration 讨论区 】
发信人: baggiosj (baggio), 信区: Immigration
标 题: 请帮忙下载几篇文章， 在线等
发信站: BBS 未名空间站 (Tue Jan 7 23:13:07 2014, 美东)
1. FGF, TGFβ and Wnt crosstalk: embryonic to in vitro cartilage development
from mesenchymal stem cells, journal of tissue engineering and regenerative
medicine.
DOI: 10.1002/term.1744
2. Enhancing human nucleus pulposus cells for biological treatment
approaches of degenerative intervertebral disc diseases: a systematic review
，journal of tissue engineering and r... 阅读全帖 |
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L*******s
发帖数: 59 | 20 我说的是观察到的现象上来说有好多是比较精确的调控，具体到microscopic层面肯定
是非常noisy的。如何在非常noisy的micro尺度得到macro尺度上的精确调控本身就是一
个很难的问题，很多人都在研究这个。这个问题不清楚，用现有的工程方法是没办法
insulate很多crosstalks和side effects。所以我建议LZ最好先不要搞太复杂的系统。
重要的是找个大牛，先发几篇好文章，等职位安全了再挑战复杂的系统。 |
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p*******l
发帖数: 10 | 21 千人彭金荣担任动物科学学院和生命科学学院两个院长, 从2008年回国后发表的SCI论
文（说明：IF标注的是2013的），包括综述。
1: Zhu Z, Chen J, Xiong JW, Peng J. Haploinsufficiency of Def activates p53-
dependent TGFβ signalling and causes scar formation after partial
hepatectomy. PLoS One. 2014 May 6;9(5):e96576. （IF=3.534）
2: Ou Z, Yin L, Chang C, Peng J, Chen J. Protein interaction between p53 and
Δ113p53 is required for the anti-apoptotic function of Δ113p53. J Genet
Genomics. 2014 Feb 20;41(2):53-62.
(IF=2.924)
3: Tao T, Shi H, Huang D, Peng J. Def... 阅读全帖 |
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s******y
发帖数: 28562 | 22 【 以下文字转载自 Joke 讨论区 】
发信人: Highly (高妹), 信区: Joke
标 题: 【相声】学术封神榜
发信站: BBS 未名空间站 (Mon May 16 23:11:52 2016, 美东)
A：现代人啊，退休都太早了。
B：六十五也不算早了吧？
A：你看人家姜子牙，找到工作的时候都七十多了，还能成就大业。
B：那他之前干嘛去了？
A：从省重点学堂出来后，状元没考中。一边给县令儿子当私家补习挣钱，复读一年，
还是不中。于是决定去昆仑山魔法学校学法术。
B：魔法学校，怎么去啊？
A：听人家说的，先来到城门口，找准一面墙。趁别人叫马车的时候，冲着那面墙冲过
去，你就进入仙界了。
B：这是封神榜啊还是哈利波特啊？
A：结果来到昆仑山后很失望，发现每天干来干去就是炼丹，种花，学数理。
B：那姜子牙在昆仑山待了多久？
A：二十年。
B：为啥这么久才毕业？
A：选错课题了呗！人家学数理的三年就毕业了，去给诸侯当推算运势的决策家，拿高
薪住大茅屋。种花的去了皇家园林，开发新品种兼写书。他炼丹的一开始倒是进展快，
也发了不少高因子论文，可到了Phase III的时候硬给FD... 阅读全帖 |
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f*******K
发帖数: 34 | 23 我知道这篇文章，Sample Multiplexing with Cysteine-Selective Approaches:
cysDML and cPILOT, 相关工作在ASMS会议报道过。他们所用的方法基本还是基于传统
方法改良如Thiopropyl Sepharose 6B resin和Cys TMT，这些基于抗体和resin方法的
富集效率比较低，从鉴定数目就可以看到。我们的富集效率可以达到97%，很轻松鉴定
到10000+ unique peptides。
目前没有同时含有iodoacetamide+phosphate的tag。phosphate可能会被phosphatase酶
切掉，phosphonate不能，因此更加稳定。
至于为什么要富集Cys peptide,因为其low abundance,essential for the biological
activity and structure, highly sensitive and reactive.
同时富集目的是研究PTM crosstalk.在我的文章里面有很好的例子，protein tyrosine
... 阅读全帖 |
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发帖数: 1 | 24 Two Postdoctoral Fellow positions are available for highly motivated and
productive applicants in the laboratory of Dr. Subhamoy Dasgupta at the
Department of Cell Stress Biology, Roswell Park Cancer Institute, NY- a
National Cancer Institute (NCI) designated comprehensive cancer center. The
Dasgupta lab will be moving to Roswell Park during the summer of 2017 and
the laboratory will be fully functional by July 2017. The laboratory focuses
on discovering novel metabolic adaptations that promote ... 阅读全帖 |
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h*******1
发帖数: 15 | 25 美国西北大学土木与环境工程系Prof. Yun Wang 招收联合培养博士生
Prof. Yun Wang from the Department of Civil and Environmental Engineering at
Northwestern University is seeking for Ph.D. candidates to conduct research
in her lab as exchange students under the joint-training programs sponsored
by China Scholarship Council (CSC). Students with educational background
in Environmental Chemistry, Microbiology, Molecular Biology, or related
majors are encouraged to apply. Successful candidates must have research
experience/i... 阅读全帖 |
|
S*********1
发帖数: 3004 | 26 平时听的最多的都是是个人都能做老师，终于有人觉得教育工作者伟大啦。
【 以下文字转载自 Parenting 讨论区 】
发信人: crosswalk (crosstalk), 信区: Parenting
标 题: 学校的一封信, 很感动
发信站: BBS 未名空间站 (Wed Aug 29 13:09:30 2012, 美东)
学校的校长助理是个50多岁的白人, 今天发了一封信, 说去年被诊断为ALS, slowly
losing the use of hands and legs, 所以以后就会坐电动轮椅出现在校园里工作了.
这个学校的孩子从2岁到12岁都有, 他在信里详细的列举了不同年龄的孩子看到他坐电
动轮椅会有什么样的问题, 家长应该怎么回答, 非常细心. 对大的孩子, 如果有兴趣的
话, 也可以和他讨论相关的神经系统的问题以及电动轮椅的机械问题. 还建议老师们可
以见缝插针的教孩子们平时碰到坐轮椅的人应该有怎样的礼仪, 他可以现身说法.
查了下这个病, 神经系统引起的肌肉无力, 目前只有药物可以延缓病情, 诊断以后的
life expectation是2-5年, 虽然有些病... 阅读全帖 |
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F****a
发帖数: 73 | 27 Help for search 3 papers IEEE. Please send to j********[email protected].
Thanks a lot !!!!!
1
Accuracy estimation of mixed-mode scattering parameter measurements
Bockelman, D.E.; Eisenstadt, W.R.; Stengel, R.;
Microwave Theory and Techniques, IEEE Transactions on
Volume 47, Issue 1, Jan. 1999 Page(s):102 - 105
2
Direct measurement of crosstalk between integrated differential circuits
Bockelman, D.E.; Eisenstadt, W.R.;
Microwave Theory and Techniques, IEEE Transactions on
Volume 48, Issue 8, Aug. |
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d*****a
发帖数: 110 | 28 Some of hot topics I'm familiar with: low power, signal integrity at nano
level, crosstalk/coupling, post-layout simulation, etc.
You can refer to the publications of DAC, ICCAD and other conferences.
Papers => fundings. |
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c*********6
发帖数: 858 | 29 Trace impedance,
Differential traces,
Layers stack up,
signal current return path,
crosstalk,
ground bounce,
Transmission line,
termination,
topology,
clock distribution,
etc. |
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g***h
发帖数: 28 | 30 有什么学科既用硬件的东西：比如power/signal integrity,crosstalk,... 又需要DSP |
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l********s
发帖数: 358 | 31 在网上看到的这两个interview问题
1. You have three adjacent parallel metal lines. Two out of phase signals
pass through the outer two metal lines. Draw the waveforms in the center
metal line due to interference. Now, draw the signals if the signals in
outer metal lines are in phase with each other.
out of phase: center metal line没有signal
in phase: center metal line的signal是和两边的一致
我不知道这样对不对，这应该是Crosstalk的问题吧
2. What happens if we increase the number of contacts or via from one metal
layer to the next?
是两层之间的r |
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c****e
发帖数: 7 | 32 PTHs(Plated Through Holes) are commonly used to connect signal traces for
internal layers (e.g. ground layer) of a PWB (Printed Wiring Board) to its
surface components. However the geometric of PTH affects the electrical
characterization through the capacitance coupling and inductance coupling (
resonating, bouncing effects etc. come from the coupling). For high speed
digital signal transmissions, the amount of crosstalk generated from the PTH
to traces must be considered as a measure of signal |
|
e***7
发帖数: 118 | 33 本人烂校PhD，方向是signal integrity，老板是美国人，快退休了，一点都不push，
我目前在找signal integrity方向的实习或者full-time，看EE版上很少有人讨论
signal integrity的，想抛砖引玉，请各位大侠都来交流交流。
先抛砖：北美signal integrity方向最牛，出的SI方向的人最多的是哪个学校？我随便一搜，大把大把SI方向的中国人都是U Missouri Rolla 出来的。
欢迎大家讨论Stephen Hall, Eric Bogatin的书，讨论jitter, crosstalk, transmission line, VNA, TDR, HFSS, ADS...... |
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T******T
发帖数: 3066 | 34 Probably need to determine the noise source 1st before your act. Is it due
to crosstalk between your high switching digital signals and or is it really
power supply related due to the high switching current sourced from the
supply as your suspicion indicates ?
If the noise indeed manifests itself on power and ground then decoupling
near the power supply would help to mitigate it. If your glitch is on the
signal only, then decoupling the supply won't help, and instead you should
try to look at p... 阅读全帖 |
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I***a
发帖数: 704 | 35 我觉得就是把layout弄好了以后，
extract出寄生参数然后仿真没有问题就可以放心了吧。
crosstalk和charge sharing在extract出寄生参数以后的仿真里都可以体现出来的。 |
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l*******0
发帖数: 9 | 36 EE master， strong background in digital logic design, VLSI and computer
architecture. 还做过SOC的interconnect方面的research（repeater insertion and
BIST for crosstalk）. 求相关公司的内部推荐， 谢谢大家！ |
|
s*****t
发帖数: 987 | 37
Glitch 分析难道没有这个报告么，不记得了
sta工具应该报告glitch crosstalk这些吧
就算route进去shield的区域里面，clock tree skew latency jitter 都满足是不是也
没啥大
问题
design flow 总是在不断改进，lz不要自责 |
|
h*******1
发帖数: 15 | 38 美国西北大学土木与环境工程系Prof. Yun Wang 招收联合培养博士生
Prof. Yun Wang from the Department of Civil and Environmental Engineering at
Northwestern University is seeking for Ph.D. candidates to conduct research
in her lab as exchange students under the joint-training programs sponsored
by China Scholarship Council (CSC). Students with educational background
in Environmental Chemistry, Microbiology, Molecular Biology, or related
majors are encouraged to apply. Successful candidates must have research
experience/i... 阅读全帖 |
|
w*******y
发帖数: 60932 | 39 Use coupon code EMCKGKJ25, $549.99 regularly minus $50 from the coupon.
Link:
http://www.newegg.com/Product/Product.aspx?Item=N82E16824009223
Great 1280x720 projector that runs at 120Hz for 2D content but really shines
when paired with Nvidia's 3D Visions kit or DLP Link glasses. The DLP
color wheel on this one adds a blank (black) frame in with the normal RGB
that displays when the LCD shutters are in transition, completely
eliminating the crosstalk that is a fact of life with LCD based 3D dis... 阅读全帖 |
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w*******y
发帖数: 60932 | 40 Thehighdefinitionsto re.com has the samsung PN59D6500 for 1855 after
special email price and call in to tell your favorite baseball team with
free white glove delivery, free samsung 2011 blu ray pack (4 shreks megamind
and 2 glasses), and free HDMI cable. Those blu rays are exclusive to
samsung and go for about $140 dollars on ebay. This is the lowest price I
have found by far for this TV. The closest through amazon is 1971 and that
does NOT include the 3d kit. The 6500 has the same picture... 阅读全帖 |
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w*******y
发帖数: 60932 | 41 Crown XL 5000 rackmount stereo amplifier for $534 shipped!
This is a barn burner price. I see this amp most places for $1k. Crown is
a well recognized and high quality manufactorer. 3 year full warranty.
This amp actually sells on ebay used for more than this new price in in all
of the most recent sales. I don't know that's it's worth flipping because
it weighs 73 pounds and by the time you pay shipping and fees you wouldn't
turn a profit - but it's nice to know you can buy something new for... 阅读全帖 |
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w*******y
发帖数: 60932 | 42 This is the lowest price for universal active 3D glasses I've seen. They are
fairly new, so not a lot of reviews.
Link:
http://www.bestbuy.com/site/SONY COMPUTER ENTNMT AMERICA - 3D GLASSES/2904117.p?id=1218459141544&skuId=2904117&st=sony 3d glasses&cp=1&lp=1
Best-in-class active 3D quality delivers full HD playback without the
resolution loss of passive 3D
Fully immersive vision universally compatible with ACTIVE 3D TVs and
exclusively supports SimulView technology
Lightweight, stylish design a... 阅读全帖 |
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m****g
发帖数: 530 | 43 Harvard Chinese Life Science Annual Research Symposium (2010)
May 29th Saturday
Jimmy Fund Auditorium
44 Binney Street, Boston, MA, 02115
Working language: Chinese/English
9:40 am: Qingsong Liu open remarks, 15 min
10:00 am ~ 12:00pm: Keynote speakers (Host: Zhang Li)
10:00 ~ 10:30am
Dr. Qiufu Ma
Associate Professor of Neurobiology, DFCI
Title: Crosstalk between pain and itch
10:30 ~ 11:00am
Dr. Cheng Li
Associate Professor of Biostatistics, DFCI
Title: Transcription factor and microRNA regu |
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b*****l
发帖数: 9499 | 44 这个问题困惑我整一年了，发出来向各位大牛求教。
当然了，套话先说一遍：一个 model 并不是 details 越多或者越少就越好，而是看多
复杂的 model 恰好可以适应这个 model 所 target 的应用。
现在切入正题：
有些组做 model 的趋势是越来越复杂和具体，横向看，越来越多的 components 被
integrate 进来，各 module 之间的 crosstalking 也越做越多；纵向看，各个
module 也越来越向 molecular level 迈进，同时越来越多地把 spatial info/
locations and diffussion/mass transport 模拟进来。
另一些组则是越做越简单，试图从大量纷繁复杂的模型中找出简单的共性来分析，或者
是从特别复杂的系统里切出一小块特别关键的部分来研究，而避免试图跟整个 system
的所有细节较劲。
我个人的倾向是 simple is beautiful，但现在一个老板一句话就让我闭嘴了：你的
model 如何给 clinical researchers 以指导？否则，如何针对具体 |
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