w***0
发帖数: 222 | 1 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 2 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 3 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 4 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 5 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 6 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 7 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 8 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 9 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 10 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 11 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
w***0
发帖数: 222 | 12 :))
遗传性的diseases, you will see those words, autosomal dominant, recessive, X
- chromsomal, mitochondrial etc. those are inheriable. However, other
diseases like Graves' Disease has genetic prodisposing factor, just like you
said, higher risk but not 遗传性的disease. |
|
y***i
发帖数: 11639 | 13 Different cancer prefers to amplify different chromsome,so he wants to
know if different chromosome has differential expression from the start
point.
I know it is not a great question but he just wants to know. I am
wondering if anyone has done something like that.
总体 |
|
s****6
发帖数: 34 | 14 Someone overexpressed a certain microRNA in a cancer cell line and then
compared the mRNA profiles of control cells vs microRNA overexpressed cells.
It seems very obvious that a bounch of genes from a certain chromsome
location were all elevated at mRNA level. Those genes are also functionally
related too, but none of them is among the predicted targets of this microRNA
according to the results from all the popular bioinformatical web-tools. What could be the mechanism underneath? What should be... 阅读全帖 |
|
Z******5
发帖数: 435 | 15 Bacterial Artificial Chromsomes，BAC？ |
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b*******0
发帖数: 48 | 16 you can directly tell if the cell is in M phase by looking at the chromosome
arrangement. Cells in M phase tend to have no nuclear membrane and
condensed chromsomes are ready to be aligned at the plate.
A good marker for cells from last S to G2 phase is CEP-F antibody. |
|
G***G
发帖数: 16778 | 17 HWI-ST594:1:2305:19270:26517#CTTGTACTTGTA 89 1 9989 23
8M1D92M = 9989 0
TTGCGTGTCGATAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC
CTAACCCTAACCCTAACCCTAACC B>DDBBBDDCA825BC@A?>BDBA;;B@EAA@DDFDA?HFHHC=
GIHFC8GIIHD?JJIJHIJIHHF?HIJHG?JIIIHEJIGGGEJHHHHHFFFDD?CB XT:A:N NM:i:4
XN:i:12 SM:i:23 AM:i:0 X0:i:1 X1:i:1 XM:i:3 XO:i:1 XG:i:1 MD:Z:0G2T0C3
^C92 XA:Z:1,-9991,2S6M1D92M,5; |
|
q****q
发帖数: 9 | 18 should be 1.
(the third field) |
|
G***G
发帖数: 16778 | 19 how to extract sequence and chromsome location from gene id
using a R package?
thanks |
|
b********e
发帖数: 95 | 20 方向：
epigenetics, mitosis, meiosis, chromsome segregation, genome stability,
yeast related genetics and genome scale screen |
|
w********a
发帖数: 324 | 21 在bacteria中，想knockin一个外来基因到chromsome中的话，最好插入到什么地方呢？
1. silent gene（通过transcriptional analysis确认的genome中没有表达的基因）；
2. Some intergenic region
3. 某tRNA 基因 rigion
谢谢！ |
|
l*******i
发帖数: 153 | 22 有意思。
是female mESC吗？是的话，先做两个实验：
1）H3K27me3 immunostaing：可能发现small dot in nucleus.
2)qRT-PCR测Xist的表达，或者用FISH。
证实的话，就说明出现X chormosome inactivation了。
几个问题：
3) mESC的培养条件？serum,还是2i/LIF?
1）knockdown的mESC是否仍保持未分化状态，还是开始分化了？如果未分化，那么很有
可能你发现了一个新的调节X reactivation/inactivation的因子，做深了，至少是
cell stem cell.如果细胞分化了，那么X chromosome上的genes表达下调不奇怪，有多
大新意取决于你的这个gene
2）看一下这个基因的功能，是否已有先关报道，与X chromosome reactivation有关。
是histone modifier, TF还是在X chromsome上的新lncRNA? |
|
i*********0
发帖数: 915 | 23 我明天确认一下是不是female 的ESCs。
对于其他的问题：
几个问题：
3) mESC的培养条件？serum,还是2i/LIF?
serum + LIF的培养条件
1）knockdown的mESC是否仍保持未分化状态，还是开始分化了？如果未分化，那么很有
可能你发现了一个新的调节X reactivation/inactivation的因子，做深了，至少是
cell stem cell.如果细胞分化了，那么X chromosome上的genes表达下调不奇怪，有多
大新意取决于你的这个gene
如果要看Oct4等gene的表达的话，RNA 水平上看到降低，Protein水平上有20%左右的降
低。但是这些pluripotency的gene在蛋白水平还维持在一个高的状态。
2）看一下这个基因的功能，是否已有先关报道，与X chromosome reactivation有关。
是histone modifier, TF还是在X chromsome上的新lncRNA?
该gene目前的功能还不清楚。另外，我看到Xist gene在shRNA处理想样品中，在RNA水
平上有3倍数的降低。 |
|
x*****e
发帖数: 309 | 24 Illumina HumanMethylation 450K BeadChip kit (Illumina, San Diego, CA, USA)
查到一篇文章里面有个我感兴趣的基因甲基化位点(CpG 位于基因的 5 UTR)， 但是我
按照文章给出的位置 （Chromsome N,position XXXXX，）去NCBI找，对应的却不是我
感兴趣的基因，相差200kb。我又找了另外几个文章中的甲基化位置，自己在NCBI上找
了找，还是对不上，不知道什么原因。 难道那篇文章参照不是NCBI的数据？还是NCBI
数据更新了？先谢了！ |
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m*********D
发帖数: 1727 | 25 借鉴以前的chromsome walking，可以找几个restriction enzymes，在你的construct
里面没有位点。用这些酶，单个酶切genomic DNA。然后链接，希望是一个片段的两端
自连。然后设计PCR primers， 从你的construct 往外拉，争取把flanking
sequeences 拉出来，再测序。理论上可行，多拭几个不同的酶。你的construct，要是
做transgenic之前要做了linearization，你的primer设计会容易得多。我没有自己做
过，就是一个idea。细节你得自己多想想。 |
|
c*****t
发帖数: 1879 | 26 即使能生下来也不能繁衍。跟骡子差不多。人和猩猩 chromsome 数不一样。 |
|
s*******r
发帖数: 181 | 27 Gene-gene interaction of imputed genome-wide data?
I have used R for heatmap, but definitely not good for 2mx2m. Split by
chromsome x chromosome may work. |
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