D*a
发帖数: 6830 | 1 嵌合鼠的“不纯”和需要回交的老鼠的不纯不是一个概念。
在基因敲除老鼠中，一般把做过基因修饰的胚胎干细胞注射到小鼠的完整胚胎中，借助
小鼠的完整胚胎进行发育。这个过程中为了区分外源胚胎干细胞和受体小鼠细胞，一般
采用两种花色的老鼠比如agouti色的129老鼠注入到黑色B6老鼠中，这样出来的是嵌合
鼠，它的每个细胞都是纯合的，或者是129纯合，或者是B6纯合，但是老鼠全身因为含
有两种品系的老鼠细胞，所以总体上不是“纯系”。
这样的老鼠，理论上生殖系统也会有这两种品系的细胞构成，因此，跟129交配，生出
来的或者是杂合的129-B6的老鼠（不带有转基因），或者是纯合的129老鼠（带有转基
因）。
做回交的老鼠，是比如上面我拿到了纯合129的转基因老鼠，我希望研究这个基因在C3H
这个品系上的应用，那么我用纯的129老鼠和C3H老鼠交配，这样的老鼠出来的每个细胞
都是不纯的，因为每个细胞都含有一条C3H的染色体和一条129的染色体。然后这样的第
一代老鼠F1继续跟C3H老鼠交配，第二代老鼠就含有多一点C3H(大约75%)，少一点129。
循环往复跟C3H老鼠交配，129越来越少，直到达到纯... 阅读全帖 |
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z*t
发帖数: 863 | 2 Beutler得奖，Medzhitov会不会有意见？
http://www.sciencedirect.com/science/article/pii/S1074761309002
Ruslan Medzhitov
This year marks the 20th anniversary of a publication (Janeway, 1989) that
in many ways revolutionized our understanding of the immune system. As part
of the proceedings of the Cold Spring Harbor Symposium on Immune Recognition
, the paper authored by the late Charles A. Janeway, Jr. was not a standard
peer-reviewed publication. In fact, Charlie used to refer to it as “the
best paper I've ... 阅读全帖 |
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h*****t
发帖数: 103 | 4 谢谢，但是有些迷糊了。
因为ATCC上面这样写的：
Description
lymphoblast, established from long-term bone marrow cultures of C3H/HeJ mice
infected with the Friend murine leukemia virus. Morphology: mostly round
cells growing singly or in small clusters in suspension, some cells are
loosely adherent.
但是，另外一个人的paper却写的该细胞为myeloid：
Shi B, Prisco M, Calin G, Liu CG, Russo G, Giordano A, Baserga R. Expression
profiles of micro RNA in proliferating and differentiating 32D murine
myeloid cells. J Cell Physiology 207: 706-10,... 阅读全帖 |
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h*****t
发帖数: 103 | 5 另外，32D来自C3H/HeJ 小鼠，为H-2k，C57/B6老鼠为H-2b，32D打到后者里面是不是应
该会被排斥掉呢？ |
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h******y
发帖数: 1374 | 6 The Nobel Prize in Physiology or Medicine 2011
shall be divided, with one half jointly to
Bruce A. Beutler and Jules A. Hoffmann
for their discoveries concerning the activation of innate immunity
and the other half to
Ralph M. Steinman
for his discovery of the dendritic cell and its role in adaptive immunity
Summary
This year's Nobel Laureates have revolutionized our understanding of the
immune system by discovering key principles for its activation.
Scientists have long been searching for the g... 阅读全帖 |
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c******a
发帖数: 267 | 7 I think the mouse line with tlr4 mutation is C3H/HeJ line, not C57/BL6 mice.
I did all my endotoxic experiments using LPS on C57/BL6 mice, how come they
are TLR4 deficient? |
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b****r
发帖数: 17995 | 8 Science. 1998 Dec 11;282(5396):2085-8.
Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4
gene.
Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell D, Alejos
E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B,
Beutler B.
Source
Howard Hughes Medical Institute and the Department of Internal Medicine,
University of Texas Southwestern Medical Center, Dallas, TX 75235-9050, USA.
mice.
they |
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l**h
发帖数: 998 | 9 我发现本版出现了军版风格, 对事实一知半解，不求甚解, 讲起故事来添油加醋, 仿佛
亲身经历，以爆"内幕八卦"为荣
Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4
Gene
我们隔壁实验室大批地用C57BL/6研究TLR4 pathway, 不知道跟C57BL/10ScCr有什么关
系.
军版的业余军迷好忽悠. 咱们这里都是内行, 说话最好讲点credit. |
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c******a
发帖数: 267 | 10 I think the mouse line with tlr4 mutation is C3H/HeJ line, not C57/BL6 mice.
I did all my endotoxic experiments using LPS on C57/BL6 mice, how come they
are TLR4 deficient? |
|
b****r
发帖数: 17995 | 11 Science. 1998 Dec 11;282(5396):2085-8.
Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4
gene.
Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell D, Alejos
E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B,
Beutler B.
Source
Howard Hughes Medical Institute and the Department of Internal Medicine,
University of Texas Southwestern Medical Center, Dallas, TX 75235-9050, USA.
mice.
they |
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l**h
发帖数: 998 | 12 我发现本版出现了军版风格, 对事实一知半解，不求甚解, 讲起故事来添油加醋, 仿佛
亲身经历，以爆"内幕八卦"为荣
Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4
Gene
我们隔壁实验室大批地用C57BL/6研究TLR4 pathway, 不知道跟C57BL/10ScCr有什么关
系.
军版的业余军迷好忽悠. 咱们这里都是内行, 说话最好讲点credit. |
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D*a
发帖数: 6830 | 13 1。 此路不通（CNS），别试了。（悲催的是，没有引用。。。）
2。看上去在这个组织中，Abcam的一抗比cell signaling的好，dilution也高，我们从
这个开始试吧。（悲催的是，没有引用。。。）
3。一篇文章某疾病模型的表现型在C3H背景的老鼠，他们发表的结果10个月的时候还是
没有表型，另一篇文章用B6的，发表的结果是从7个月开始的，那么看来我们的老鼠应
该在7个月左右就有表型了。 （悲催的是，不一定引用。。。）
4。某博后发了一篇（nature）protocol的文章，世界上无数实验室拿这篇文章开始做
实验。。。（悲催的是，被评价为，技术文章，没啥看头。。。）
5。xx et al 发在Nature，yy et al发在plos bio，zz et al发在JBC上的文章，同时
证明了，protein A在BBB中的重要作用。（悲催的是。。。）
我认为科研的评价系统需要改改了。
欢迎补充。 |
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l********s
发帖数: 70 | 14 C3H/10T1/2 是不是细胞系 in the database of
commonly misidentified cell lines maintained by ICLAC
非常感谢！ |
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a****y
发帖数: 1035 | 15 【 以下文字转载自 Biology 讨论区 】
发信人: hellowhy (夹死do it), 信区: Biology
标 题: 2011 Nobel Prize in Physiology or Medicine
发信站: BBS 未名空间站 (Mon Oct 3 08:44:48 2011, 美东)
The Nobel Prize in Physiology or Medicine 2011
shall be divided, with one half jointly to
Bruce A. Beutler and Jules A. Hoffmann
for their discoveries concerning the activation of innate immunity
and the other half to
Ralph M. Steinman
for his discovery of the dendritic cell and its role in adaptive immunity
Summary
This year's Nobel Laureate... 阅读全帖 |
|
