wh
发帖数: 141625 | 1 cia的tourist就是杀手的意思？搞得那么诗意。eco的书啃起来很难啊，你花了多少时
间看完prague cemetery的？他的风格其实挺传统，背景介绍多，洋洋洒洒，适合长途
旅行时带到船上看一两个月，就像以前的人看安娜卡列尼娜一样。
真奇怪，我记得去年发圣诞读书包子帖，你列了一长串书单。那些读书帖都找不到了，
是我记错了还是都删了。倒是找到你前年的读书总结：
发信人: bos (Bart), 信区: LeisureTime
标 题: 2010年终读书总结
发信站: BBS 未名空间站 (Thu Dec 30 13:35:49 2010, 美东)
到年底了，回头看看总结今年看过的几本书。感觉上今年大部分时间都泡在水里了，当
然，这完全都是Patrick O'Brian的缘故。他的Aubrey-Maturin系列，总共二十本，今
天已经看到第十七本了。所以，航海应该算是今年的主题。
好几年前，粗略看过这个系列的第一本书，《Master and Commander》，没有什么感觉
。当时好像是Russell Crowe的同名电影刚上映，就把书拿过来翻翻。当时英语不好，
说实话，没有... 阅读全帖 |
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i*********t
发帖数: 5873 | 2 references。。
发信人: wh (wh), 信区: WebRadio
标 题: Re: 来学习波哥的讲话
发信站: BBS 未名空间站 (Wed Oct 7 14:07:37 2009, 美东)
他说的原文是这篇，很长：
发信人: iamwhatieat (我吃故我在), 信区: Reader
标 题: Fascinating Fascism
发信站: BBS 未名空间站 (Thu Oct 1 02:19:18 2009, 美东)
Fascinating Fascism
by Susan Sontag
New York Review of Books
February 6, 1975
republished in:
Under the Sign of Saturn
(New York, 1980), pp. 73-105.
First Exhibit. Here is a book of 126 splendid color photographs by Leni
Riefenstahl, certainly the most ravishing book of phot... 阅读全帖 |
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b*s
发帖数: 82482 | 3 好像就是诺曼底和bulge惨点，总体吃亏不大
★ 发自iPhone App: ChineseWeb 7.8 |
|
P****e
发帖数: 389 | 4 奇怪的果实
—— Abel Meeropol
南方的树上结着奇怪的果实，
带血的根系，带血的叶子。
黑色的身体，摇荡于南国的微风。
奇怪的果实从杨树上垂落。
壮丽的南方，牧歌中的风景——
扭曲的嘴唇、凸出的眼睛。
玉兰树的花香，新鲜甜美。
忽如其来，有一阵炙肉的焦腥。
看这些果实
供乌鸦饮啄，供大雨漂聚，供大风吸吮，
由树顶高垂，供太阳腐蚀——
这奇怪的、苦涩的粮食。
Strange Fruit
Southern trees bear strange fruit
Blood on the leaves and blood at the root
Black bodies swinging in the southern breeze
Strange fruit hanging from the poplar trees.
Pastoral scene of the gallant south
The bulging eyes and the twisted mouth
Scent of magnolias, sweet and fresh
Then the sudden smell o... 阅读全帖 |
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p***r
发帖数: 20570 | 5 Strange Fruit
Southern trees bear strange fruit 南方的树长奇怪的果子，
Blood on the leaves and blood at the root 树叶和根浸泡在血中。
Black bodies swinging in the southern breeze 黑色的尸体在南风中飘
荡，
Strange fruit hanging from the poplar trees. 奇怪的果子从杨树枝头
垂下。
Pastoral scene of the gallant south 华丽南方那（田园）牧
歌的景象，
The bulging eyes and the twisted mouth (掩不住）凸起的眼睛和
扭曲的嘴。
Scent of magnolias, sweet and fresh 木兰的香气，甜蜜而新
鲜，
... 阅读全帖 |
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P****e
发帖数: 389 | 6 这个比我还实译，赞～ 只是那句Pastoral scene from the gallant South, 我觉得这
个scene指的就是下句 the bulging eyes and twisted mouth，充满了讽刺，而不是＂
掩不住＂的意思。待会儿去听听你推荐的We shall overcome。
子， |
|
w***s
发帖数: 15642 | 7 一．蒲公英
“从草地上的蒲公英就能看出这家的房子状况。我敢打赌，对面那家的房子维护得比眼
前这家好。”说这话的是我们的房产经纪，一个四十多岁的精明胖妇人。我们随着她的
指点看去，果然大有区别。昨夜刚下过雨，蒲公英绒球都被打散，眼前这家的草地上到
处戳着一根根光杆儿；马路对面的草地却像一块泛着光泽的绿天鹅绒，连个毛刺都没有。
和我们一样，蒲公英是欧亚大陆的来客。它的英文名dandelion，源自法文dent-de-
lion，形容其叶有锯齿，如同狮子牙。孙思邈在《千金要方》里称它为“凫公英”，指
它的果实像野鸭子颈上的绒毛。严格来讲，中国和美国的蒲公英并非同种，但都属于菊
科的蒲公英属(Taraxacum)。最早把它引进北美的人，也许是看中它清肝解毒的药用价
值。北美最常见的Common dandelion，学名Taraxacum officinale，这里officinale就
特指药用植物。
今日，蒲公英是北美庭院最常见的野草，其药用价值早已被大多数人忽略，为了杀死蒲
公英而喷洒的杀草剂成为主要水污染源。也有不信邪的，有位国人朋友喜爱它嫩叶的美
味，把后院种满了蒲公英。一年下来，满地... 阅读全帖 |
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b*s
发帖数: 82482 | 8 guns of navarone
the longest day
battle of the bulge
today is Veteran's day
any war movie to recommend |
|
d*******d
发帖数: 3382 | 9 The 'Tiger Mom' Superiority Complex
A new book from Amy Chua and Jed Rubenfeld seeks to explain why some groups
succeed in America, and some fail. But when does cultural pride cross over
into racism?
By Suketu Mehta Monday, Feb. 03, 2014
From time to time, every Indian American finds an email in his or her inbox,
wearing a font of many colors, like the one my grandfather once sent me: "
Take a Pride--Being an Indian. 38% of Doctors in U.S.A. are Indians. 36% of
NASA employees are Indians. 34% of... 阅读全帖 |
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i**i
发帖数: 2042 | 10 我先说我的买的：
WallE
A bug's life
Kungfu Panda
Caroline
The Shawshank Redemption
The Shining
Brave Heart
Save Private Ryan
Casino Royale
Blade Runner
Independence Day
2012
Forrest Gump
Unforgiven
Sin City
The Patriot
Battle of the Bulge
Band of Brothers
Planet Earth
Winged Migration
Wild China
HOME
Celine Dion A new Day |
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b*s
发帖数: 82482 | 11 那个是Battle of the Bulge…… |
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u*******s
发帖数: 688 | 12 请看原链接。。。
http://www.mitbbs.com/article_t/LeisureTime/1261855.html
不知道配什么乐，本来是想用巴赫的无伴奏大提琴，选来选去没有特别合适的。
Strange Fruit
Southern trees bear strange fruit
Blood on the leaves and blood at the root
Black bodies swinging in the southern breeze
Strange fruit hanging from the poplar trees.
Pastoral scene of the gallant south
The bulging eyes and the twisted mouth
Scent of magnolias, sweet and fresh
Then the sudden smell of burning flesh.
Here is fruit for the crows to pluck
For the rain to gather, for th... 阅读全帖 |
|
x****y
发帖数: 1853 | 13 Really? 不过 21 zm 跟24L不好比吧。 广角差一毫米差很远的。 更何况是旁轴和单反
的比较。
Ken大嘴的评价，且当笑话看看：
Distortion performance top
The Zeiss ZM 21/2.8 has no visible distortion. This is better than the LEICA
ELMARIT-M 21mm f/2.8 ASPH or Voigtländer 21mm f/4. The Voigtlä
nder has a little barrel distortion, and the LEICA ASPH has a bit of bulging
distortion in the center of its image. Even shooting brick walls, this
Zeiss ZM 21 2.8 is flat. |
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l*******G
发帖数: 1191 | 14 This is ZT from http://blogs.timesofindia.indiatimes.com/extraordinaryissue/entry/the-industry-of-lies
The question is :
Why do we lie so much in this world to be able to succeed? What's wrong?
Have you noticed how we have, in recent times, institutionalised the art of
cheating? In fact, we have built so many successful enterprises around it
that we have now actually forgotten why these industries first came up.
Botox was initially not about enhancing our looks, as we have now come to
believe... 阅读全帖 |
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a*****c
发帖数: 3525 | 15 【 以下文字转载自 Military 讨论区 】
发信人: agostic (π-packing), 信区: Military
标 题: (中英文)原子弹下无冤魂：B29飞行员查尔斯•斯韦尼将军1995国会演讲
发信站: BBS 未名空间站 (Wed Aug 15 20:13:42 2012, 美东)
只有记忆才能带来真正的原谅，而遗忘就可能冒重复历史的危险。
One can only forgive by remembering. And to forget, is to risk repeating
history.
学英文是为了更好地了解历史。文章很长，有兴趣学英语同时了解历史的同学，希望耐
心地看完（也可以拷贝下来仔细研读）。不仅仅是学英语，更重要的是学如何写作，如
何摆事实，讲道理，反驳质疑。可以学的东西(中英文)原子弹下无冤魂：B29飞行员查
尔斯•斯韦尼将军1995国会演讲很多，就看你是否能够用心去领悟。这是一篇难
得的informative/historical/linguastic article. Enjoy and welcome to ... 阅读全帖 |
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b*s
发帖数: 82482 | 16 德国人还是比日本人文明很多。虽然二战的时候有很多暴行，但是整体上还算是比较文
明的。
1944后期和45年初，有人向希特勒献计：运用神风突击队性质的自杀攻击来对付盟军，
希特勒认为这个太疯狂了，没有采纳。希特勒虽然给世界带了了巨大的灾难，但是对于
用自杀攻击的这个方面，还算是有底线的。
当时，Battle of the Bulge里面，德国人出现了杀俘现象。其实美军也有杀俘现象，
很多战史里面都有记载。有一个据点的一个班的美军凭借地形狙杀了上百德军，最后的
残余被抓住了，愤怒的德军士兵准备杀俘，被德国军官阻止了。德军里面也有很多
honorable的fighter……
"It's tempting to see your enemies as evil -- there was good and evil on
both sidesin every war ever fought."
日军比较之下就很野蛮，完全没有文明规则。 |
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b*s
发帖数: 82482 | 17 美军在battle of the bulge里面，对于穿美军制服的德国特种兵，也是就地正法的。
游击队和穿对方制服的，都当作间谍处理，就地正法。间谍是不受日内瓦公约保护的。 |
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b*s
发帖数: 82482 | 18 其实最后ss也没遭严惩。在battle of the bulge里面杀俘的Joachim peiper, 一开始
被判死刑，但是由于美军审讯的时候逼供，最后死刑取消了。
后来他的ss 死党还在大众给他谋了个差事，还翻译英文战记。最后是去法国买地定居
以后，被以前的抵抗分子杀了…… |
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z****6
发帖数: 10776 | 19 还是不知道怎么得到的结果
http://en.wikipedia.org/wiki/Gravity_of_Earth
In combination, the equatorial bulge and the effects of the Earth's inertia
mean that sea-level gravitational acceleration increases from about 9.780 m
·s−2 at the Equator to about 9.832 m·s−2 at the poles, so an
object will weigh about 0.5% more at the poles than at the Equator |
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r****z
发帖数: 12020 | 20 原标题：五维黑洞“黑环”可能存在：或颠覆广义相对论(图)
文章来源: 网易科技 于 2016-02-24 20:03:20
在科学家们的脑海中，他们认为宇宙中可能还存在着一种非常特殊类型的黑洞，即所谓
“黑环”
所谓“奇点”是指一个特殊的点，在这个点上，由于引力强度达到无限大，空间、时间
以及物理学定律本身都将失效。广义相对论禁止裸奇点的存在
科学家们相信这种神秘的黑环可能存在于宇宙之中，并且其维度可以达到5维甚至更高
。近日，一项关于5维黑洞的最新研究结果让我们得以更进一步了解这类特殊黑洞的一
些性质
北京时间2月25日消息，据英国《每日邮报》报道，黑洞的存在已经随着引力波信号的
直接观测再次得到了确凿无疑的证明。而在科学家们的脑海中，他们认为宇宙中可能还
存在着一种非常特殊类型的黑洞，即所谓“黑环”(black ring)。
科学家们相信这种神秘的黑环可能存在于宇宙之中，并且其维度可以达到5维甚至更高
。近日，一项关于5维黑洞的最新研究结果让我们得以更进一步了解这类特殊黑洞的一
些性质。
参与相关研究的科学家们表示，如果这类特殊黑洞的确存在，那么它将导致“裸奇点”
的存在，从而最终... 阅读全帖 |
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c****c
发帖数: 102 | 21 鄙人年方26，身长6脚2寸=184.5cm，天朝北方人士，本布衣，奈何挣扎于藤校，后出，
入NYC某Bulge Bracket钱庄百日有余，友人云：高大威猛+成熟稳重+知识丰富，成熟稳
重中不乏幽默风趣，遂今征通情达理，有气质之mm，共求彼此之进步。
无他要求。古人云，有缘千里来相会；非诚勿扰，可站内信。 |
|
c**i
发帖数: 6973 | 22 (1) Amir D Aczel, An Expedition Without End; Battling subzero temperatures,
swarming mosquitoes and angry Inquisitors, all to find a single number. Wall
Street Journal, June 27, 2011
http://online.wsj.com/article/SB10001424052
702304186404576387761564776914.html
(book review on Larrie D. Ferreiro, Measure of the Earth; The Enlightenment
Expedition that Reshaped the World. Basic Books, 2011)
Quote: "But Newton's recently published theory of mechanics indicated that a
spinning Earth would look mor... 阅读全帖 |
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C*******l
发帖数: 1198 | 23 Going Nowhere
Indeed the passion is gone
Grief mutes into a hushed tone
Flowers fall into lesser fruits
Battles bulge into more burials
This singular second of serenity
Of nothingness, of heat, of rot, of joys, of lies,
Of anything I long for and detest,
Melts down into an empty concept of both past and future,
Taking away any possibility of a lucid vision of present.
Suddenly I become the enemy of myself
Before memories fades
Before Tears evaporate into salts
Before Emotions petrifies into sile |
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x****g
发帖数: 342 | 24 As my eyes fully adjust to the afternoon sun, I finally find her at the
other side of the arena. I start to jog to her around the edge of the arena
so I won’t be surrounded as easily. My gaze returns to my opponents. They
are almost as thinly built as we are which is rarely the case. They all
wearsimilar armor and all wear helmets that cover half of their faces and
their heads, so I can’t identify them unless I move closer. Then I notice
the bulge in their chest armor.
They’re gladiatrices; fema... 阅读全帖 |
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c****t
发帖数: 19049 | 25 HALF-LIVES
the first luisa rey
mystery
I
Rufus Sixsmith leans over the balcony and estimates his body’s velocity
when it hits the
sidewalk and lays his dilemmas to rest. A telephone rings in the unlit room.
Sixsmith dares not
answer. Disco music booms from the next apartment, where a party is in full
swing, and
Sixsmith feels older than his sixty-six years. Smog obscures the stars, but
north and south
along the coastal strip, Buenas Yerbas’s billion lights simmer. West, the
Pacific eternity East... 阅读全帖 |
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h******v
发帖数: 1826 | 26 windows are not air tight...could feel a draft near the window...the cold
air leaks around the windows and into the room...
the carpet seam is visible and part of it is bulging |
|
h******v
发帖数: 1826 | 27 windows are not air tight...could feel a draft near the window...the cold
air leaks around the windows and into the room...
the carpet seam is visible and part of it is bulging |
|
w***r
发帖数: 7173 | 28 windows are a little bit leaky (or not air tight).
there are places on carpet where cutting lines or bulging are visible. |
|
e*******e
发帖数: 325 | 29 普利兹奖的得主，芭芭拉·泰克曼(Barbara Tuchman)，这位历史学家坚持写历史
的重要原则就是不可改变时间的顺序，当她写到第二次世界大战盟军和德军在巴尔吉(
Bulge)的最后决战时，她必须拒绝写下“当然，我们都知道这场战役的结果”这样句子
的引诱。因为事实上，当时在参与巴吉格之役的盟军并不知道战争结果是如何。根据那
时候的情况，他们很可能是被逼回他们登陆的诺曼底海滩上。一个历史学家如果要想保
持述说外观上的紧张和戏剧性，他就必须放弃已经知晓结局的立场，这样才能有那种身
临其境的感受，否则，便会失去戏剧性。能够带着读者回到当初的情境之中，得到感同
身受的滋味的历史学家，才是一个好的历史学家。
我的结论就是，我们大部分有关耶稣的著作和想法都犯了上述的毛病，我们在读福
音书的时候，总是带着尼西(Nicea)或是迦列敦(Chalcedon)镜片，﹣﹣(自以为)已经知
道结果就是教会一直努力研究想要透彻地搞懂他。
耶稣是一个人，是一个有名有姓、有家有世，与你我一样的一个人，他是一个出生
在加利利的犹太人。可是从另外一方面来说，耶稣是和地上任何一个活过的人完全不同
。早期教会花... 阅读全帖 |
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J*******g
发帖数: 8775 | 30 这是老文章了。我看过N遍了。
这里说的religiou不见得是基督教。
murder rate在religious states低，并不是说基督徒中muder rate高。监狱里无神论
者少，不见得跟基督徒有关。即使有关也许人家是进去后信的呢？
“知道你对正经的文章没兴趣”只能说明你不了解我乱说而已。我习惯了。
“Admittedly, when it comes to underage alcohol consumption or illegal drug
use, secular people do break the law more than religious people (Benson 1992
; Gorsuch 1995; Hood et al. 1996; Stark and Bainbridge 1996). But when it
comes to more seri-ous or violent crimes, such as murder, there is simply no
evidence suggesting that atheist and sec... 阅读全帖 |
|
J*******g
发帖数: 8775 | 31 不如你来读读，也许你的阅读能力更好呢，看看你的文献能不能支持你的观点：
“现今的基督教：
受教育程度越高，信的越少。
在美国犯罪率超过无神论者不止一个数量级。”
“Admittedly, when it comes to underage alcohol consumption or illegal
druguse, secular people do break the law more than religious people (Benson
1992; Gorsuch 1995; Hood et al. 1996; Stark and Bainbridge 1996). But when
itcomes to more serious or violent crimes, such as murder, there is simply
no evidence suggesting that atheist and secular people are more likely
to commit such crimes than religious people. After a... 阅读全帖 |
|
J*******g
发帖数: 8775 | 32 不如你来读读，也许你的阅读能力更好呢，看看你的文献能不能支持你的同伴的观点：
“现今的基督教：
受教育程度越高，信的越少。
在美国犯罪率超过无神论者不止一个数量级。”
“Admittedly, when it comes to underage alcohol consumption or illegal
druguse, secular people do break the law more than religious people (Benson
1992; Gorsuch 1995; Hood et al. 1996; Stark and Bainbridge 1996). But when
itcomes to more serious or violent crimes, such as murder, there is simply
no evidence suggesting that atheist and secular people are more likely
to commit such crimes than religious people. Afte... 阅读全帖 |
|
g***l
发帖数: 3142 | 33 帅哥如潮，美女如云。
简要情况：
纽约哈佛俱乐部，酒会+晚宴
大概去了160-170人
>95% professionals
>95% in finance industry
>70% in Bulge Brackets
>50% with rank of VP or higher
~40% math or stat at PKU
~40% physics at PKU
大家很高兴啊
到晚上11店多才散 |
|
x****g
发帖数: 342 | 34 The Show down
Darkness engulfs me. My body tenses up and I tell myself to relax. The shaft
of my spear is wet with the sweat of anticipation and fear coming down my
stiff hands. I take a deep breath and think about the peaceful times I have
had with my beautiful and elegant partner in the forest meadow and stream
just relaxing and forgetting about what we actually have become...
The speech of a senator comes indistinctly through the trap door that
separates me and the bloody arena. It is probabl... 阅读全帖 |
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s*******g
发帖数: 1607 | 35 Have you found indentured servitude to be rewarding, or are there drawbacks?
Do you guys have Coke or Pepsi in your vending machines? 'Cause
Pepsi ain't gonna cut it.
Want to arm wrestle for an offer?
Can I receive my signing bonus in SEPTA tokens please?
Speaking of bulge-bracket banking... do you think this suit is too tight?
Do you want a twinkie? No, I insist... come on, there are like 20 in this box.
Really, I can't eat them all.
Care to stop by Au Bon Pain for a monopoly pricing model case |
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L*******s
发帖数: 15925 | 37 【 以下文字转载自 LeisureTime 讨论区 】
发信人: wells (墨酥), 信区: LeisureTime
标 题: 【交作业】野草
发信站: BBS 未名空间站 (Thu Jul 25 02:26:50 2013, 美东)
一．蒲公英
“从草地上的蒲公英就能看出这家的房子状况。我敢打赌，对面那家的房子维护得比眼
前这家好。”说这话的是我们的房产经纪，一个四十多岁的精明胖妇人。我们随着她的
指点看去，果然大有区别。昨夜刚下过雨，蒲公英绒球都被打散，眼前这家的草地上到
处戳着一根根光杆儿；马路对面的草地却像一块泛着光泽的绿天鹅绒，连个毛刺都没有。
和我们一样，蒲公英是欧亚大陆的来客。它的英文名dandelion，源自法文dent-de-
lion，形容其叶有锯齿，如同狮子牙。孙思邈在《千金要方》里称它为“凫公英”，指
它的果实像野鸭子颈上的绒毛。严格来讲，中国和美国的蒲公英并非同种，但都属于菊
科的蒲公英属(Taraxacum)。最早把它引进北美的人，也许是看中它清肝解毒的药用价
值。北美最常见的Common dandelion，学名Taraxacum officinal... 阅读全帖 |
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a***y
发帖数: 19743 | 38 H.264 VGA video, 640 by 480 pixels, up to 30 frames per second with AAC
audio
15 real-time special effects
Sepia, Black and White, X-Ray, Film Grain, Thermal, Security Cam, Cyborg,
Bulge, Kaleido, Motion Blur, Mirror, Light Tunnel, Dent, Stretch, and Twirl
还不错
不知道能不能拍照啊？
也许不能。 |
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w****2
发帖数: 12072 | 39 Stevey's Google Platforms Rant
I was at Amazon for about six and a half years, and now I've been at Google
for that long. One thing that struck me immediately about the two companies
-- an impression that has been reinforced almost daily -- is that Amazon
does everything wrong, and Google does everything right. Sure, it's a
sweeping generalization, but a surprisingly accurate one. It's pretty crazy.
There are probably a hundred or even two hundred different ways you can
compare the two companies... 阅读全帖 |
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w****2
发帖数: 12072 | 40 用google translate做个简单翻译：
Stevey的谷歌平台的咆哮
我在亚马逊是约6年半，现在我已经在谷歌这么久。一件事我立即袭击有关两家公司 -
已加强几乎每天都有一个印象 - 是亚马逊的一切错误，和谷歌的一切权利。当然，它
的一概而论，但令人惊讶的准确。这实在是太疯狂。有可能是一个百年甚至两百年不同
的方法可以比较两家公司，谷歌是优越的，但他们三人，如果我没有记错。其实我在一
个点上的电子表格，但法律不会让我展示给任何人，即使招募喜欢它。
我的意思，只是给你一个非常简短的味道：亚马逊的招聘过程基本上是由队聘请为自己
，所以他们雇用栏是令人难以置信的团队之间不一致的，尽管他们所做的水平出来各种
努力，有缺陷的。他们的行动是一个烂摊子，他们并不真正有国营事业
，他们的工程师几乎做的一切，这让几乎没有时间进行编码 - 虽然这组不同，所以它
的幸运抽奖。他们不给一个慈善或帮助有需要的人或社会的贡献或类似的东西的单狗屎
。从未出现在那里，除了也许笑。他们的设施是污垢弄脏没有装饰或共同会议领域上花
一毛钱的立方体农场。他们的薪酬和福利吸，虽然少得多，所以... 阅读全帖 |
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s******o
发帖数: 298 | 41 Invisible corona
The stars of the Milky Way's main disk span a region of space about 100,000
light-years across, and the thick central bulge is a sphere about 25,000
light-years in diameter. To picture this, think of a typical sci-fi flying
saucer. For decades, this was the official picture of our galaxy. More
recently, a handful of studies have added a sprinkling of stars that live in
a sparse halo outside the main disk.
Then early this year, astronomers made a remarkable announcement: Our gala |
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s*****t
发帖数: 1994 | 42 Counting Stars in the Infrared Sky
Explanation: The bulging center of our Milky Way Galaxy, dark cosmic clouds, the thin
galactic plane, and even nearby galaxies are easy to spot in this sky view. But each pixel in
the digital image is actually based on star counts alone -- as derived from the Two Micron
All Sky Survey (2MASS) database. In 2001, the 2MASS project completed a ground-based
survey of the entire sky and cataloged upwards of 250 million stars. Their full all-sky picture
assigns a bri |
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c*****t
发帖数: 1084 | 43 since i'm in this field i think sth should be clarified. so far
the only functional miRNAs has been found in worms and plant
but not in mammlian cells although miRNAs are detected in mammalian
cells. no targets have been found yet. also in worms miRNA (actually
let-7 and lin-4) is not perfectly complenentary to its target
seq at 3'UTR. some bulges exist withink base paring region. this
posts most difficult part to identify potential miRNA target
seq since it's not perfectly match. also miRNA is |
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a****o
发帖数: 1786 | 44 I did not read all posts. I guess LZ is doing SELEX. What he/she refered is
the bulge structure formed by many different molecules share two constant
regions at both ends, but with different sequences in the middle.
The simplest way to solve this problem is to dilute your sample to 4 fold
after PCR, add do ONE more cycle of PCR. |
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