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发帖数: 991 | 1 https://www.addgene.org/57818/
In the comment, it was suggested that "Use BsmBI sites for sgRNA cloning.
Note that this plasmid does NOT contain the 1.9kb stuffer from the pLKO.005
vector backbone." But I couldn't find BsmBI site on the plasmid map....
Also what is the sequencing primer to confirm a successful cloning of sgRNA
into this vector?
Thanks. |
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