s********l
发帖数: 1195 | 1 J Biomol Screen. 2001 Apr;6(2):83-90.
HTPS flow cytometry: a novel platform for automated high throughput drug
discovery and characterization.
Edwards BS, Kuckuck FW, Prossnitz ER, Ransom JT, Sklar LA.
Thanks! |
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o******e
发帖数: 284 | 2 if>1
1 CHEM REV 40.197
2 NAT MATER 32.841
3 CHEM SOC REV 28.760
4 ACCOUNTS CHEM RES 21.640
5 NAT CHEM 20.524
6 ALDRICHIM ACTA 16.091
7 NANO TODAY 15.355
8 ANNU REV PHYS CHEM 14.130
9 ADV MATER 13.877
10 ANGEW CHEM INT EDIT 13.455
11 NANO LETT 13.198
12 COORDIN CHEM REV 12.110
13 SURF SCI REP 11.696
14 ACS NANO 10.774
15 MED RES REV 10.700
16 J PHOTOCH PHOTOBIO C 10.360
17 ADV FUNCT MATER 10.179
... 阅读全帖 |
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b******n
发帖数: 4225 | 3 1. 抗体的选择
对于国内的大多数实验室来讲，做western blot实验选择抗体是个头疼的问题。原因很
简单，买进口抗体捉襟见肘，买国产抗体得需要大无畏的勇气，对于我所在的兰州地区
的实验者而言，感触尤深。在 这五年的western blot实验历程里，我先后用过进口抗
体，进口抗体国内分装包装，国产抗体，质量良莠不齐。
进口抗体一般不会出现闪失：abcam品种全，质量过硬，但价高（3400元/100微升），
而且说是100微升，但至多能吸出来90微升；Sigma的 价最贵；比较有性价比的是CST的
抗体，现在好像是2200元/100微升，我前后用过二十几种， 1:1000的稀释比下，还没
有失过手，100微升通常能完成所有的免疫组化和western blot实验，还有一个优点是
通常量比100微升多出来10微升，唯一的不足是CST的品种实在不多。Santa的多克隆抗
体质量可以，但是选用 Santa的单抗还是有风险，估计这也是业界共识了吧。
进口抗体国内分装包装我也用过不少，呵呵，毕竟是穷人（420元/100微升），大概好
抗体的比例约为50%，如果能做出来，也存在一个问题，就是... 阅读全帖 |
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i****g
发帖数: 3896 | 4 写得比较有意思，但是不太实用，可能实验条件相差太大。precast gel比自己制的gel
效果要好很多，结果也容易重复。antibody要看运气的，呵呵。
1. 抗体的选择
对于国内的大多数实验室来讲，做western blot实验选择抗体是个头疼的问题。原因很
简单，买进口抗体捉襟见肘，买国产抗体得需要大无畏的勇气，对于我所在的兰州地区
的实验者而言，感触尤深。在 这五年的western blot实验历程里，我先后用过进口抗
体，进口抗体国内分装包装，国产抗体，质量良莠不齐。
进口抗体一般不会出现闪失：abcam品种全，质量过硬，但价高（3400元/100微升），
而且说是100微升，但至多能吸出来90微升；Sigma的 价最贵；比较有性价比的是CST的
抗体，现在好像是2200元/100微升，我前后用过二十几种， 1:1000的稀释比下，还没
有失过手，100微升通常能完成所有的免疫组化和western blot实验，还有一个优点是
通常量比100微升多出来10微升，唯一的不足是CST的品种实在不多。Santa的多克隆抗
体质量可以，但是选用 Santa的单抗还是有风险，估计这... 阅读全帖 |
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w********h
发帖数: 12367 | 5 【 以下文字转载自 Biology 讨论区 】
发信人: bulletin (bulletin), 信区: Biology
标 题: 穷人的劳斯莱斯——五年western经验谈（转）
发信站: BBS 未名空间站 (Fri Mar 2 23:29:05 2012, 美东)
1. 抗体的选择
对于国内的大多数实验室来讲，做western blot实验选择抗体是个头疼的问题。原因很
简单，买进口抗体捉襟见肘，买国产抗体得需要大无畏的勇气，对于我所在的兰州地区
的实验者而言，感触尤深。在 这五年的western blot实验历程里，我先后用过进口抗
体，进口抗体国内分装包装，国产抗体，质量良莠不齐。
进口抗体一般不会出现闪失：abcam品种全，质量过硬，但价高（3400元/100微升），
而且说是100微升，但至多能吸出来90微升；Sigma的 价最贵；比较有性价比的是CST的
抗体，现在好像是2200元/100微升，我前后用过二十几种， 1:1000的稀释比下，还没
有失过手，100微升通常能完成所有的免疫组化和western blot实验，还有一个优点是
通常量比100微升多出来10... 阅读全帖 |
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d******6
发帖数: 276 | 6 HI blehum
I'd like review MS for JAPS.
pls see the following information. Many thanks
Ⅰ: PERSONAL INFORMATION
Name: Xiaoyi XU
Telephone Number: +1-(650)391-5991
E-mail Address: d******[email protected];
x**[email protected]
Address: Stanford University, School of Medicine
3801 Miranda Ave, Palo Alto, CA, 94304, US
Ⅱ: EDUCATION EXPERIENCE
YEAR INSTITUTION DEGREES /
2003.09-2006.06 Changchun Institute of Applie... 阅读全帖 |
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d******6
发帖数: 276 | 7 我是做高分子化学药物控释，包括前药这个方向的，求审稿推荐。多谢
Ⅰ: PERSONAL INFORMATION
Name: Xiaoyi XU
Telephone Number: +1-(650)391-5991
E-mail Address: x**[email protected]
Address: Jilin University
Stanford University, School of
Medicine
3801 Miranda Ave, Palo Alto, CA, 94304, US
Ⅱ: EDUCATION EXPERIENCE
YEAR INSTITUTION DEGREES /
2003.09-2006.06 Changchun Institute of Applied Chemistry, Ph... 阅读全帖 |
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t******y
发帖数: 716 | 8 IP目的蛋白后，用anti-Ub的抗体，western检测。或者直接纯化目的蛋白，用in vitro
Ubiquitination assay检测，biomol有卖in vitro的试剂盒。 |
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a********k
发帖数: 2273 | 10 JCR Year and Edition: 2010 Science
Abbreviated Journal Title ISSN {2010} Total Cites Impact Factor
5-Year Impact Factor Immediacy Index {2010} Articles Cited Half-
Life Eigenfactor Score Article Influence Score
CA-CANCER J CLIN 0007-9235 9801 94.262 70.216 8.667 18
3.8 0.04923 24.782
ACTA CRYSTALLOGR A 0108-7673 13944 54.333 24.717 0.629 70
6.2 0.04... 阅读全帖 |
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h*****x
发帖数: 147 | 11 Qun Li,Chaoyu Chen,Amit Kapadia,Qiong Zhou,Mary Kay Harper,Jerome Schaack,
Daniel V. Labarbera
3D Models of Epithelial-Mesenchymal Transition in Breast Cancer Metastasis:
High-Throughput Screening Assay Development, Validation, and Pilot Screen
J Biomol Screen 2011 16: 141-154
http://jbx.sagepub.com/content/16/2/141.full.pdf+html
email: h************[email protected]
Thanks so much! |
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S***a
发帖数: 257 | 12 pls send to columxia at gmail.com
Thank you and happy new year!
Evaluation of the carbohydrate recognition domain of the bacterial adhesin
FimH: design, synthesis and binding properties of mannoside ligands Oliver
Sperling, Andreas Fuchs and Thisbe K. Lindhorst
Org. Biomol. Chem., 2006, 4, 3913-3922 |
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Q**********r
发帖数: 214 | 13 A Chemical Genomics Screen to Discover Genes That Modulate Neural Stem Cell
Differentiation
J Biomol Screen. 2011 Sep 23. [Epub ahead of print]
please send it a*************[email protected]
thanks a lot! |
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l**********1
发帖数: 5204 | 14 Do not tell me you can not understand
the upper space journal and bottom space journal their IF difference.
CR Year and Edition: 2010 Science
Abbreviated Journal Title ISSN {2010} Total Cites Impact Factor
5-Year Impact Factor Immediacy Index {2010} Articles Cited Half-
Life Eigenfactor Score Article Influence Score
CA-CANCER J CLIN 0007-9235 9801 94.262 70.216 8.667 18
3.8 0... 阅读全帖 |
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Q**********r
发帖数: 214 | 15 J Biomol Screen. 2010 Aug;15(7):830-9. Epub 2010 Jul 16.
Knockdown of cancer testis antigens modulates neural stem cell marker
expression in glioblastoma tumor stem cells.
please send to a*************[email protected]
不胜感激! |
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z***q
发帖数: 907 | 16 J Biomol NMR. 1991 Nov;1(4):391-402.
Effects of internal motions on the development of the two-dimensional
transferred nuclear Overhauser effect.
Thanks a lot for any help! |
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z***q
发帖数: 907 | 17 A heteronuclear correlation experiment for simultaneous determination of 15N
longitudinal decay and chemical exchange rates of systems in slow
equilibrium.
Farrow, N. A.; Zhang, O. W.; Forman-Kay, J. D.; Kay, L. E., J Biomol NMR
1994, 4, 727-734.
Many thanks! |
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y******8
发帖数: 1764 | 18 Abbreviated Journal Title ISSN {2012} Total Cites Impact Factor
5-Year Impact Factor Immediacy Index {2012} Articles Cited Half-
Life Eigenfactor Score Article Influence Score
CA-CANCER J CLIN 0007-9235 13722 153.459 88.55 27.04 25
3.3 0.0517 29.478
NEW ENGL J MED 0028-4793 245605 51.658 50.807 12.667 360
8 0.65776 21.494
REV MOD PHYS 0034-6861 35720 44.982 51.882 6.478 46 10
0.13048 32.634... 阅读全帖 |
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w******e
发帖数: 1187 | 19 我的email: [email protected]
/* */ 非常感谢！！
paper 1:
title: Multi-Specific Antibodies for Cancer Immunotherapy
BioDrugs, August 2014, Volume 28, Issue 4, pp 331-343
Ron D. Jachimowicz, Sven Borchmann, Achim Rothe
paper 2:
title: New Horizons in Therapeutic Antibody Discovery: Opportunities and
Challenges versus Small-Molecule Therapeutics
J Biomol Screen April 2015 20: 437-453
paper 3:
title: Non-immunoglobulin scaffolds: a focus on their targets
Katja Škrlec, Borut Štrukelj, Ale#... 阅读全帖 |
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q****e
发帖数: 3660 | 23 Efficient conversion of triacylglycerols and fatty acids to biodiesel in a
microwave reactor using metal triflate catalysts
EIX 10-42 E20104113284819 NDN- 267-0893-5071-9
ABBREVIATED JOURNAL TITLE- Org. Biomol. Chem.
VOLUME 8
NUMBER 20
PUBLICATION DATE- 2010-10-21
Thanks! |
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x********u
发帖数: 390 | 25 1. Y.Z. Xu and E.T. Kool, Tetrahedron Lett., 1997, 38, 5595-5598
2.El-Sagheer AH, Cheong VV, Brown T.,Rapid chemical ligation of
oligonucleotides by the Diels-Alder reaction, Org Biomol Chem. 2011 Jan 7;9(
1):232-5.
3. Sergei M. Gryaznov, Robert L. Letsinger, Chemical ligation of
oligonucleotides in the presence and absence of a template, J. Am. Chem. Soc
., 1993, 115 (9), pp 3808–3809
Thank you a lot for your help!!! |
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x********u
发帖数: 390 | 26 1. Y.Z. Xu and E.T. Kool, Tetrahedron Lett., 1997, 38, 5595-5598；http://www.sciencedirect.com/science/article/pii/S0040403997012665
2.El-Sagheer AH, Cheong VV, Brown T.,Rapid chemical ligation of
oligonucleotides by the Diels-Alder reaction, Org Biomol Chem. 2011 Jan 7;9(
1):232-5. http://pubs.rsc.org/en/content/articlelanding/2011/ob/c0ob00451k
3.Sergei M. Gryaznov, Robert L. Letsinger, Chemical ligation of
oligonucleotides in the presence and absence of a template, J. Am. Chem. Soc
., 1993, 11... 阅读全帖 |
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y****z
发帖数: 92 | 28 Can someone please download this paper for me? Thank you!
A convenient method for selective detection of 5-hydroxymethylcytosine and 5
-formylcytosine sites in DNA sequences
Org. Biomol. Chem., 2013,11, 3568-3572 |
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u**s
发帖数: 384 | 29 Title:A novel method for selective isotope labeling of bacterially expressed
proteins
Authors:Lee KM1, Androphy EJ, Baleja JD.
Journal:J Biomol NMR. 1995 Jan;5(1):93-6.
Link:http://www.ncbi.nlm.nih.gov/pubmed/7881274
Thanks! |
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u**s
发帖数: 384 | 30 Title:A novel method for selective isotope labeling of bacterially expressed
proteins.
Journal:J Biomol NMR. 1995 Jan;5(1):93-6.
Authors:Lee KM1, Androphy EJ, Baleja JD.
Link:http://www.ncbi.nlm.nih.gov/pubmed/7881274
Title:Assignment of amino acid type in 1H-15N correlation spectra by
labeling with 14N-amino acids.
Journal:J Magn Reson B. 1994 Sep;105(1):88-90.
Authors:Shortle D.
Link:http://www.ncbi.nlm.nih.gov/pubmed/7921674
3x! |
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c******6
发帖数: 928 | 31 "Insight into the Binding Modes and Inhibition Mechanisms of Adamantyl-based
1,3-disubstituted Urea Inhibitors in the Active Site of the Human Soluble
Epoxide Hydrolase", J. Biomol Struct & Dyn, 31(8)(2014)1231-1247. |
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y****z
发帖数: 92 | 32 Can someone please download this paper for me? Thank you very much!
"ent-Kaurane-based regio- and stereoselective inverse electron demand hetero
-Diels–Alder reactions: synthesis of dihydropyran-fused diterpenoids "
Org. Biomol. Chem., 2014, Advance Article |
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a*****c
发帖数: 3525 | 33 我也来一稿多投，赫赫。
2003 JCR Science Edition
rank/ journal title/ impact factor
1 MASS SPECTROM REV 7.364
2 PROG NUCL MAG RES SP 5.971
3 NMR BIOMED 3.333
4 J AM SOC MASS SPECTR 3.321
5 J ANAL ATOM SPECTROM 3.200
6 J MASS SPECTROM 2.875
7 RAPID COMMUN MASS SP 2.789
8 J BIOMOL NMR 2.420
9 INT J MASS SPECTROM 2.361
9 SPECTROCHIM ACTA B 2.361
11 J MAGN RESON 2.084
12 APPL SPECTROSC 1.717
13 ANNU REP NMR SPECTRO 1.696
14 J ANAL APPL PYROL 1.463
15 SOLID STATE NUCL MAG 1.453
16 J |
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p**l
发帖数: 10 | 34 Anyone would like to help find the articles?
1. Drug Discovery Today: Technologies, Volume 1, Issue 4, December 2004,
Pages 365-372
"Metabolic screening in vitro: metabolic stability, cyp inhibition and
induction."
Author: Robert J. Riley and Ken Grim
2. J Biomol Screen. 2007 Feb;12(1):106-16.
"High-throughput bioluminescence screening of ubiquitin-proteasome pathway
inhibitors from chemical and natural sources."
Authors: Ausseil F, Samson A, Aussagues Y, Vandenberghe I, Creancier L,
Pouny I, Kr |
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p**l
发帖数: 10 | 35 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: ppll (matched2008), 信区: Pharmaceutical
标 题: Help with Article
发信站: BBS 未名空间站 (Tue Feb 12 09:21:51 2008)
Anyone would like to help find the articles?
1. Drug Discovery Today: Technologies, Volume 1, Issue 4, December 2004,
Pages 365-372
"Metabolic screening in vitro: metabolic stability, cyp inhibition and
induction."
Author: Robert J. Riley and Ken Grim
2. J Biomol Screen. 2007 Feb;12(1):106-16.
"High-throughput bioluminescence screening of ubiquitin-proteaso |
|
p**l
发帖数: 10 | 36 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: ppll (matched2008), 信区: Pharmaceutical
标 题: Help with Article
发信站: BBS 未名空间站 (Tue Feb 12 09:21:51 2008)
Anyone would like to help find the articles?
1. Drug Discovery Today: Technologies, Volume 1, Issue 4, December 2004,
Pages 365-372
"Metabolic screening in vitro: metabolic stability, cyp inhibition and
induction."
Author: Robert J. Riley and Ken Grim
2. J Biomol Screen. 2007 Feb;12(1):106-16.
"High-throughput bioluminescence screening of ubiquitin-proteaso |
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