r****o
发帖数: 105 | 1 I have similar experiences as yours. That is about determination
the anomericity of glycosylation on EGF repeats. It involves
processing the small domain EGF into peptides, and then subject them
to glucosidase digestion.
I would suggest you using HPLC with either Reverse Phase or Gel filtration
column(like superdex from Pharamacia). This is the best way to recover
small amount of peptides. If your sample has quite a lot and the MW is
big enough, you can try acetone precitation to get rid of |
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w**q
发帖数: 43 | 3 Just a thought.
For ester alone, s-cis form is favored(anomeric, A1,2 and dipole).
After addn of AlCl3, for Me, the s-trans...AlCl3 could be stable since Me is
small. But for Et, both s-cis...AlCl3 and s-trans...AlCl3 are unstable
complex due to steric effect. s-cis...AlCl3 is ready for IM cleavage of Et. |
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e******y
发帖数: 106 | 5 sent. 这个review时间上比较靠前了.
anomeric effect最近有两篇文章值得注意.
Nature Volume:469,Pages :76–79Date published:(06 January 2011) DOI:doi:
10.1038/nature09693
Nature Chemistry Volume:2,Pages:666–671 Year published:(2010)DOI:doi:10
.1038/nchem.721 |
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